Research Of Real-time PCR Technique Evaluating The Effect Of The Hepatitis B Virus Contamination Silicone Impression Disinfection

[Objective] Through Real-time PCR detects two molding silicone impressions disinfected before and change three different time points after the number of copies of HBV immersion disinfection, to evaluate three chemical disinfectants for HBV artificial saliva pollution disinfection silicone impression. In selection of the appropriate disinfectants, disinfection time in clinic. Clinical carry silicone impression disinfection experimental basis.[Methods] 1. Prepare a 2% glutaraldehyde; 1% net gifted chlorine; cavicide disinfectant neutralizer; press disinfectant neutralizer chemical identification principle, the three chemical disinfectants neutralizer identified, screened 3 chemical disinfectants against HBV artificial saliva contaminated silicone impression sterilization experiments effective neutralizer.2. Application of Real-time PCR evaluation of chemical disinfectants on two silicone impression disinfection, sterilization effect is certainly the choice of two clinical chemical disinfectants (2% glutaraldehyde; 1% gifted chlorine net) and a new chemical disinfectants (Cavicide disinfectant) for both carrying the hepatitis B virus artificial saliva contaminated with silicone impression (3M plus silicone rubber; GC addition silicone rubber) for disinfection. After setting three different points disinfection time reaches the proper time, the disinfectant is added to the disinfectant neutralizers, disinfection terminate the reaction. The extraction of the silicone rubber remaining HBV-DNA, use Real-time PCR for HBV-DNA was amplified by before and after comparison of HBV-DNA copy number sterilization, disinfection efficiency disinfectant draw different points in time. Quantitative evaluation of different disinfectants at different time points in Hepatitis B virus inactivation.[Results] 1. Identification of chemical disinfectants neutralizing experiment to give Real-time PCR evaluation neutralizer identification in chart.2.Real-time PCR in the evaluation of three kinds of disinfectants efficiency, Cavicide in 6 minutes disinfectant can inactivate hepatitis B virus surface of the silicone rubber impression at 99.9%; 1% gifted chlorine net in 10 minutes to inactivate silicone impression hepatitis B virus surface 99.4%; 2% glutaraldehyde in 35 minutes to inactivate hepatitis B virus surface of the silicone rubber impression 99.6%. Cavicide disinfectant immersion disinfection 6 minutes can effectively kill the hepatitis B virus,2% glutaraldehyde kill the hepatitis B virus, the effective time of 35 minutes,1% net gifted chlorine to kill the hepatitis B virus valid for 10 min.[Conclusion] 1. Hepatitis B virus contaminated silicone impression sterilization experiments, by neutralizing the qualification test. Select 0.5%glycine+1% Lecithin +1% Tween-80PBS as 2% glutaraldehyde neutralizing agent,0.5%Sodium thiosulfate as 1% net chlorine neutralizer,65 g/1 Tween-80+10 g/1 lecithin+10 g/l glycine PBS buffer as Cavicide neutralizer.2. Real-time PCR to assess three disinfectant before and after HBV-DNA copy number was found, Cavicide 6 minutes disinfectant can inactivate hepatitis B virus surface of the silicone rubber impression at 99.9%; 1% gifted chlorine net 10 minutes off living with hepatitis B virus surface silicone impression 99.4%; 2% glutaraldehyde 35 minutes to inactivate hepatitis B virus surface of the silicone rubber impression 99.6%. One new type of disinfectant Cavicide 99.9% over 6 minutes to inactivate the virus. Sterilization time is short, so the new disinfectant Cavicide is desirable impression disinfectant.