Stable Silence Of AQP5Gene Down-regulated Proliferation And Migration Of Ectopic Ndometrial Glandular Pithelial Cells In Endometriosis

Objective:To observe Aquaporin5(AQP5) effect on proliferation and migration of ectopic endometrialepithelialcells (EM cells) from endometriosis.Method:AQP5shRNA interference fragments were designed and transfected into ectopic endometrial epithelial cells stably by lentivirus technology. Fluorescence quantitative reverse transcription(RT) PCR and western blotting were used to detect the AQP5mRNA and protein expression. The cell proliferation,migration were determined by using methylthiazolyltetrazolium(MTT) and transwell culture system. Levels of phosphorylated AKT(p-AKT) and total AKT were examined by western blotting.The nude mice model was constructed and tumor growth and migration were observed.Results:The proliferation of AQP5shRNA trasfected cells was slower than that in negative control group(P<0.05); cell migration in shAQP5-3shRNA transfection group was lower than that in the negative control group (P<0.05).The activation of AKT in AQP5shRNA trasfected cells was lower than that in negative control group cells(P<0.01).Compared to nude mice inoculated with non-transfected cells, tumor growth and migration of endometriosis was suppressed in mice inoculated with ectopic endometrial epithelial cells stably transfected with AQP5shRNA.Conclusion:Down-regulation of AQP5expression can suppress the proliferation and migration of ectopic endometrial epithelial cells. AQP5may be involved in the biological function of ectopic endometrial epithelial cells by AKT pathway. Down-regulation of n may help to suppress the endometriosis growth and migration of lesions in mice.