Diagnostic Value Of Immunohistochemistry Staining With Anti-HPV6B/11E7Protein Polyclonal Antibody In Suspected Condyloma Acuminatum

Objective:The incidence of condyloma acuminatum(CA) is still increasing year by year. For CA with typical clinical presentation, diagnosing is not difficult. But for some CA which have neither typical clinical presentation nor typical Pathological presentation, to diagnose is not that easy. Thus, we used immunohistochemistry staining with anti-HPV6b/11E7Protein polyclonal antibody for direct detection of HPV6b and11E7protein in all the samples, observed the quantity and distribution of expression positive cells within the epidermal layer. So the object of this study was to evalute the contribution of immunohistochemical stain technique in the diagnosis of CA by using these two antibodies.Methods:We collected41paraffin-embedded lesion biopsy samples from SRRSH during2011.1to2014.8. All the lesions were diagnosed by histopathology, they were all from genital area and had no typical clinical presentation.We reviewed the clinical information of these patients, including age, gender and the clinical presentation of the lesions. HPV6b and11E7protein were detected by anti-HPV6b/11E7protein polyclonal antibodies by the application of immunohistochemistry. The number and distribution of expression positive cells for each sample were recorded.Results:Using immunohistochemistry for the detection of HPV6b and11E7protein in tissue, we found that of expression of E7protein was in the cell nuclear of full layer epidermis, and more in the spinous layer and the basal layer. HPV6bE7protein was expressed in34cases(positive rate:82.93%), HPV11E7protein was expressed in27cases(positive rate:65.85%). The two types of HPV E7protein were detected positive in38cases(positive rate92.68%), in23cases both two types of E7protein were expressed(positive rate:56.10%), and3cases were detected negative for both two E7proteins(negative rate:7.32%).Among these41cases, there were12cases whose Pathological presentation was not typical. Immunohistochemistry result showed that HPV6bE7protein was expressed in10cases(positive rate:83.33%), HPV11E7protein was expressed in6cases(positive rate:50.00%). The two types of HPV E7protein were detected positive in11cases(positive rate91.67%), in5cases both two types of E7protein were expressed(positive rate:41.67%), only1case was detected negative for both two E7proteins(negative rate:8.33%).Discussion:In this study, immunohistochemistry staining with anti-HPV6b/11E7Protein polyclonal antibody for direct detection of HPV6b and11E7protein was used for the diagnosis of CA. The positive rate of HPV6bE7protein was82.93%, and the positive rate of HPV11E7protein was50.00%.This result consisted with the result of PCR test.The positive rate of the two types of HPV E7protein was92.68%, which also consisted with the result of PCR test. Among these41cases,34cases were positive for HPV6bE7protein and27cases were positive for HPV11E7protein. There were11cases which was positive for HPV6bE7while negative for HPV11E7, and4cases were negative for HPV6bE7while positive for HPV11E7. It indicated that these two proteins had no cross-reactivity with each other.The result showed that the self-prepared anti-HPV6b/11E7protein polyclonal antibodies are highly sensitive and specific for detecting these two E7proteins in CA. Among12cases who has no typical Pathological presentation, the positive rate of the two types of HPV E7protein was91.67%. Indicate that using immunohistochemistry for the detection of HPV6b and11E7protein in tissue can improve the accuracy of diagnosis in CA especially in suspected CA.In this study, the positive rate is much higher than immunohistochemistry staining with anti-HPV L1protein monoclonal antibody. Comparing with other molecular diagnostic techniques, immunohistochemistry is cheaper and the operation of immunohistochemistry is less complicated.Conclusions:1. The self-prepared anti-HPV6b/11E7protein polyclonal antibodies are highly sensitive and specific for detecting these two E7proteins in CA.2. Comparing with other molecular diagnostic techniques, this test method is cheaper and the operation of immunohistochemistry is less complicated.3. Using immunohistochemistry for the detection of HPV6b and11E7protein in tissue can improve the accuracy of diagnosis in CA especially in suspected CA.4. The IHC using anti-HPV6b/11E7protein polyclonal antibodies is a hopeful test for the clinical practice.