Expression Of14-3-3ζ In The Progress Of Breast Cancer

Breast cancer is a common malignant tumor in women. Over the past few decades, encouraging trend in breast cancer incidence has been observed, making it rank the second place among female caners in China. The Wellings-Jensen model of human breast cancer progression proposes a linear multi-step process which initiates as usual ductal hyperplasia (UDH), progresses to atypical ductal hyperplasia (ADH), evolves into ductal carcinoma in situ (DCIS) and culminates in the potentially lethal stage of invasive ductal carcinoma (IDC). However, the key molecular events to identify tumor-stage-specific signature of the molecular alterations have not been deeply explored.Recent reports have suggested that the molecular alterations in DCIS and IDC show much consistence. The link between histological differentiation and biomarkers keeps consistent in DCIS and IDC. Such diversity expands from DCIS to IDC, fitting with the theory of breast cancer progression. And it is speculated that the molecular events highlights in the early phase of tumorigenesis, while the advanced stages focuses on tumor cells invasion and metastasis events.The14-3-3proteins comprise a family of regulatory molecules widely expressed in multiple tissues. Over-expression of14-3-3ζ is an early event in several kinds of cancers. And14-3-3ζ overexpresses in more than40%of breast cancers. Moreover, studies have shown that14-3-3ζ protein expression is dramatically increased at ADH and DCIS stages of breast disease. And in vitro studies revealed that14-3-3ζ overexpression promoted epithelial to mesenchymal transition (EMT), and disrupted acini architecture in mammary epithelial cells (MEC). These findings provide new ideas for our study.We have experienced some rare cases in which the patient got more than one breast histological types including UDH, ADH, DCIS and even IDC. These lesions of different histological types in one patient share the same genetic and environmental background, stimulating the progression of breast ductal carcinoma. In such model, the studies of molecular alteration mechanism of breast cancer progression may be more scientific and convincing.ObjectiveTo investigate the expression of14-3-3ζ in the tumorigenesis and development of breast cancer.MethodThe expression of14-3-3ζ was detected by Immunohistochemistry in6cases of breast cancer tissues. The expression levels of14-3-3ζ protein in different histological types were analyzed.Result14-3-3ζ expression was profoundly increased at the stage of ADH compared with UDH and normal mammary tissue.ConclusionBased on the six special cases, this study found that14-3-3ζ played important roles in breast carcinoma.