Effects Of High Frequency Electric Stimulation Of Habenutar Nucleus To Respiratory Movement And Cardiovascular System In Rats

Obstructive sleep apnea OSA is a high complexity and individualitydisease.It is a kind of syndrome with the features of apnea,decliningoxyhemoglobin saturation and sleep disorder which have frequentlyoccured in sleep.The gold standard for OSA diagnosis is polysomnographPSG.OSA is basically caused by breath and upper airway open disorderwhich leading to upper airway complete or incomplete obstruction andfinally manifest as apnea and hypopnea.OSA prevalence rate in adults isabout4%,untreated OSA patients’5years case fatality rate can up to11%13%.Sudden death risk has increased in untreated OSApatients.About3000people dead in a day by OSA all over the world. Theexcitation of insular cortex and habenula nucleus can cause OSA andlimbic system has important regulating effect on respiratory movement andcardiovascular system. The key to cure OSA is to interdict habenulanucleus and reduce habenula nucleus activity. High frequency electricstimulation interdict habenula nucleus is a fresh way on OSA treatment.Objective:According to the observe the variation of genioglossus myoelectricity,respiratory movement, artery blood pressure,and heart rate whichinfluenced by high frequency electric stimulation on habenular nucleus.Tofind a way to interdict the formation of rats’ OSA which has provide aproof and a method on animal experiments for OSA treatment.Methods:The90experimental rats were randomly divided into8groups by the means of random numbers. Among them, there are ten rats in the shamstimulation of insular cortex group,10rats in the stimulation of habenularnucleus at1mm group, as the control group. As to the model group, thereare12rats in the stimulation guide cortex model group, and12rats in thestimulation of habenular nucleus model group. The rest46rats were treatedas the experimental group, that is,50Hz stimulation group,120Hzstimulation group,130Hz stimulation group and280Hz stimulation group.The cortex was implanted with same sex circle electrodes in the insularcortex of rats. The respiratory pause was induced in the cortex of theinsular cortex, and the tissue was verified to establish the rat OSA model.Through the observation of different frequency stimulation habenularnucleus OSA rat model of the curve of respiration, breathing rate,breathing amplitude, systolic arterial pressure, diastolic arterial pressure,heart rate, genioglossus electromyogram (EMG) amplitude and integral,diaphragmatic EMG amplitude and integral, electrocardiogram(ECG), anddata observation analysis, the tissue samples were taken for statisticalanalysis and application SAS9.4statistical software on income data,P<0.05for statistical significance.Results:1.The breathing rate, breathing amplitude, systolic blood pressure,arterial blood pressure, and heart rate were not significantly compared withthe experimental groups before the experiment, P>0.05.2.Comparing with the control group, the breathing rate, breathingamplitude, arterial systolic pressure, arterial diastolic pressure and heartrate of model group were significantly different, P<0.001.3.There were significant differences between the130Hz stimulationgroup and the model group. P<0.001; There were significant differencescompared with280Hz stimulation group and the model groups,P<0.05. Conclusion:130Hz stimulation model of OSA rat habenular nucleus transmitted bystimulation of insular cortex nerve impulses can be blocked, genioglossusmuscle contraction can be induced and block the formation of OSA.