Study On The Preparation Of Acanthopanax Dispersible Tablet

Acanthopanax senticosus is the dried root and rhizome or stem ofAcanthopanax senticosus (Rupr.et Maxim.) Harms, and is one of the most populartraditional Chinese medicine, widely for symptoms of weak spleen, bad appetite,fatigue and insomnia. In recent years, a large number of experiments and researchesshow that Acanthopanax senticosus contains glycosides, flavonoids, polysaccharidesand trace elements; the phenolic glycosides are the main component, accounting for0.6%~1.5%of the total weight of dried herbs, with anti fatigue and antioxidant andsedative pharmacological effects. In this experiment, at first we studied the optimumextraction process of Acanthopanax, and then prepared it into dispersible tablets withfast absorption, high bioavailability, which can be directly to swallow, but also can bequickly disintegrated in the water, especially suitable for the patients with difficultyin swallowing.Firstly, we optimized the conditions of extracting from Acanthopanax by ethanoland water sequencelly, with value of expectation of eleutheroside B, E andisofraxidin as indicator. In the ethanol extracting, we studied three factors, namelyextraction time, solvent-to-solid ratio and solvent concentration. Then, we used theresponse surface methodology (RSM) with a three factor-three coded levelBox-Behnken design (BBD). The optimal extracting conditions were as follows:solvent-to-solid ratio was12.3, extraction time was2.65h, the value of D was0.6090.The relative error between Validation tests and predictive value was1.01%. Then, weoptimized the conditions of water extracting by orthogonal test, namely extractiontime, times and solvent-to-solid ratio. The optimal conditions were as follows:extracting for3times (each for2h), times and solvent-to-solid ratio was6.Secondly, we studied the preparation of Acanthopanax dispersible tablet, and setup the quality standard. We mainly investigated filler, disintegrating agent, wettingagent, taste masking agent and other factors, and finally the optimum prescription byorthogonal test was: the content of drμg was accounting for25%, the content ofCMS-Na was accounting for5%, the content of PVPP was accounting for25%, the content of L-HPC was accounting for30%. Then we establish stable and feasiblestable and feasible quality standard for Acanthopanax dispersible tablet to ensure itsquality.Finally, we studied on the preliminary Pharmacodynamics of Acanthopanaxdispersible tablets, mainly on the effects of anti fatigue, anti oxidant and sedation.The final results show that: compared with the blank group, the low dose group couldsignificantly increase swimming time of mice (p<0.01); high dose group cansignificantly reduce the spontaneous activity (p<0.01). In the hypoxia toleranceexperiment, the three dose groups can significantly improve the hypoxia tolerancetime (p<0.05); middle dose and high dose groups could significantly increase theacute hypoxia tolerance time (p<0.05), compared with the blank group. In the studyof the biochemical index, low and middle dose groups could significantly increasethe content of LDH in serum of mice (p<0.05), low、medium and high dose groupscould significantly increase the content of SOD in serum of mice (p<0.05, p<0.01,p<0.01) and decreased the content of MDA (p<0.05, p<0.01, p<0.01), compared withthe blank group. In this experiment, Acanthopanax dispersible tablets showedantifatigue, antioxidant and sedation activities.