Study On The Preparation Of Cuttlebone-transformed Hydroxyapatite And Its Properties

As raw materials, Cuttlebone, whoes main chemical composition is calcium carbonate, crystal structure is aragonite phase occurred hydrothermal reaction with diammonium phosphate under specific conditions, the final hydrothermal products were characterized respectively by XRD, FT-IR, EDS and SEM. The results showed that, the final hydrothermal product was hydroxyapatite, that was the cuttlebone-transformed hydroxyapatite (CBHA). Preparative CBHA, whose perpendicular to the grain size of the (211) crystal plane, D211was12.5nm, and kept good3D porous structure.Using universal testing machine to test the compressive strength of Cuttlebone, HA and CBHA, According to the results:the compressive strength of CBHA was greater than the compressive strength of cuttlebone, cuttlebone was greater than HA, but the compressive strength of CBHA was different with body’s natural bone.The biodegradability of CBHA were studied by SSBF and DSBF test, the results showed that, the DSBF pH declined faster than SSBF pH, HA and CBHA soaked in SBF will produce acid degradation products spreaded into SBF solution. Compared with materials soaking in SSBF, materials in DSBF degraded more quickly. The surface of HA and CBHA deposited calcium phosphate containing carbon after soaked in SBF.Studied on cytocompatibility of materials by MG63cell culture experiments in vitro and MTT test, the results confirmed that CBHA materials had no obvious effect on cell growth and no obvious toxicity, they did not affect the cell proliferation.Zinc-doped cuttlebone-transformed hydroxyapatite (Zn-CBHA), Titanium-doped cuttlebone-transformed hydroxyapatite (Ti-CBHA) and Zinc and Titanium co-doped cuttlebone-transformed hydroxyapatite (TiZn-CBHA) were prepared by hydrothermal reaction and ion exchange method. The composition, crystal shape and crystallinity, structure of the samples were characterized using EDS, XRD and FT-IR; Moreover, the bacterial inhibition zone and antibacterial ratio were tested to investigate the antibacterial properties of antibacterial cuttlebone-transformed hydroxyapatite to Escherichia coli and Staphylococcus aureus. The results indicated that, Antibacterial cuttlebone-transformed hydroxyapatite can were prepared by hydrothermal reaction and ion exchange method, Zn-CBHA, Ti-CBHA and TiZn-CBHA had good antibacterial properties to Escherichia coli and Staphylococcus aureus, the synergistic effect of the Titanium photocatalytic decomposition antibacterial and Zinc ion dissolve out antibacterial, which enhanced the antibacterial property of TiZn-CBHA.From mechanical property, biodegradability, cytocompatibility, antibacterial property comprehensive studied, The cuttlebone-transformed hydroxyapatite which is expected to become a type comprehensive performance ideal bone repair materials in the field of bone repair materials.