Study On Interleukin-correlation Factor Of Chronic Hepatitis B With Nephrasthenia Syndrome

ObjectiveStudy on the protein expression characteristics of interleukin-4(IL-4), interleukin-6(IL-6),interleukin-10(IL-10), interferon-y (IFN-y) and tumor necrosis factor a (TNF-a) of Chronic Hepatitis B(CHB) with nephrasthenia syndrome, and comprehensive analysis of HBV markers, HBV-DNA, liver function, etc, explore the molecular mechanism of CHB infection with nephrasthenia syndromeMethodsSerum specimen that clinical unit collected from CHB patients with syndrome of deficiency of kidney-YANG, kidney-YIN, symptomless and healthy control volunteers was detected by enzyme-linked immunosor bent Assays (Elisa), observed differences in protein expression levels between groups. Analyzed the count data and measurement data in SPSS17.0softwareResults1、ALT and AST in CHB patients with syndrome of deficiency of kidney-YANG, kidney-YIN and asymptomatic have no significant difference (P>0.05), but ALT, AST in kidney-YIN deficiency patients is the highest in the three groups, followed by kidney-YANG deficiency patients, asymptomatic patients is the lowest;the viral load in kidney-YANG deficiency patients, kidney-YIN deficiency patients and asymptomatic patients have no significant difference (P>0.05), but that in kidney-YANG deficiency patients and kidney-YIN deficiency patients is significantly higher than asymptomatic patients, and kidney-YANG deficiency patients is higher than kidney-YIN deficiency patients; HBeAg positive rate in kidney-YIN deficiency patients and asymptomatic patients have significant difference(P<0.05), and kidney-YIN deficiency patients is the highest, followed by kidney-YANG deficiency patients, asymptomatic patients is the lowest.2、IL-4in CHB patients is lower than healthy control, the difference is significant (P<0.01), and IL-4in healthy control, kidney-YANG deficiency patients, asymptomatic patients and kidney-YIN deficiency patients decreased in the order. IL-4in kidney-YANG deficiency patients and kidn-ey-yin deficiency patients are significantly different from healthy control (P<0.01), IL-4in kidney-YIN deficiency patients and healthy control are significantly different from asymptomatic patients (P<0.01), IL-4in kidney-YANG deficiency patients are significantly different from kidney-YIN deficiency patients (P<0.01).3、IL-6in CHB patients is lower than healthy control, the difference is significant(P<0.05), and IL-6in healthy control, asymptomatic patients, kidney-YANG deficiency patients and kidney-YIN deficiency patients in the order. IL-6in kidney-YANG deficiency patients and kidney-YIN deficiency patients are significantly different from healthy control (P<0.05), IL-6in kidney-YANG deficiency patients and kidney-YIN deficiency patients are significantly different from asymptomatic patients(P<0.05),IL-6in kidney-YANG deficiency patients are significantly different from kidney-YIN deficiency patients (P<0.05).4、IL-10in CHB patients is lower than healthy control, the difference is significant(P<0.01),and IL-10in healthy control, kidney-YANG deficiency patients, asymptomatic patients and kidney-YIN deficiency patients decreased in the order. IL-10in kidney-YANG deficiency patients and kidney-YIN deficiency patients are significantly different from healthy control (P<0.01), IL-10in kidney-YIN deficiency patients and healthy control are significantly different from asymptomatic patients (P<0.05), IL-10in kidney-YANG deficiency patients are significantly different from kidney-YIN deficiency patients (P<0.05).5、IFN-y in CHB patients is lower than healthy control, the difference is significant (P<0.01), and IFN-γ in healthy control, kidney-YANG deficiency patients, asymptomatic patients and kidney-YIN deficiency patients decreased in the order. IFN-y in kidney-YANG deficiency patients and kidney-YIN deficiency patients are significantly different from healthy control (P<0.05), IFN-y in kidney-YIN deficiency patients and healthy control are significantly different from asymptomatic patients (P <0.05), IFN-y in kidney-YANG deficiency patients are significantly different from kidney-YIN deficiency patients (P<0.01).6、TNF-a in CHB patients is lower than healthy control, but the difference is not significant (P>0.05), and TNF-α in healthy control, kidney-YANG deficiency patients, asymptomatic patients and kidney-YIN deficiency patients decreased in the order. TNF-α in kidney-YIN deficiency patients are significantly different from healthy control(P <0.05), kidney-YIN deficiency patients are significantly different from asymptomatic patients (P<0.05).7、Analysis ALT, HBV-DNA, HBeAg with IL-4, IL-6, IL-10, IFN-y and TNF-a found that, with the increasing of ALT, IL-4valuegradually decreased, but L-6, IL-10, IFN-y and TNF-a have no obvious trend. HBV-DNA have no correlation with IL-4, IL-6, IL-10, IFN-y and TNF-α;IL-4, IL-6, IL-10, IFN-γ and TNF-a in HBeAg negative patients were significantly higher than that in HBeAg positive patiens. Conclusion1、Analysis CHB patients relevant clinical indexes,syndrome of kidney deficiency patients with liver damage may be more heavy than asymptomatic patients, CHB patients with syndrome of deficiency of kidney-YANG and kidney-YIN is invalid.2、Thl cells dominated, causing inflammation and cell injury, Th2cells dominated, prone to persistent chronic infection. IL-4, IL-10areanti-inflammatory factor, can inhibit Thl cell proliferation and response. IL-4, IL-10in CHB patients with syndrome of deficiency of kidney-YIN are lower than kidney-YANG deficiency patients, suggest that kidney-YIN deficiency patients liver inflammation and injury degree is heavier than kidney-YANG deficiency patients; as ALT in kidney-YIN deficiency patients, kidney-YANG deficiency patients, asymptomatic patients increased, IL-4decreased gradually, provides a basis for kidney-YIN deficiency patients liver inflammation and injury degree is heavier than kidney-YANG deficiency patients from the angle of liver function. IL-6, IFN-y and TNF-a can inhibit Th2cell proliferation and respons, IL-6, IFN-y and TNF-a in kidney-YIN deficiency patients are lower than kidney-YANG deficiency patients, suggest that the probability of persistent infection of kidney-YIN deficiency patients may be higher than kidney-YANG deficiency patients.