The Influence Of Repair And Dynamic CTGF Expression After The Early Electric Injury Of Bile Duct By High Blood Sugar Condition Of Rabbit

Objective:The rabbit ear vein injection of alloxan hyperglycemia animal model in rabbits,to observe the extent and scope of the early damage under the ordinary light microscope of a certain intensity of a certain time certain parts of the coagulation of the bile duct after modeling,to observe the pathological process of hyperglycemia on bile duct tissue repair and bile duct wall electrocautery organization in existence and what impact effect by the expression intensity of CTGF.Though the hyperglycemic state rabbits and normal rabbit bile duct injury model in the early self repair of bile duct wall pathological changes and CTGF expression was observed in the dynamic process of determination of comparison,provide the related experimental research data for the treatment of diabetic patients with bile duct injury.Materials and Methods:Establish hyperglycemia model,66New Zealand white rabbits using random number table divided into control group and model group,modeling group36,control group30rats,the control group were fed with normal diet, model group induced by rabbit ear vein injection of alloxan.The fasting blood glucose (FBG)≥11.0mmol/L as the standard model.Establishment of bile duct electric burn model, control group and model group using random number table divided into electrocoagulation injury after1d group,3d group,5d group,7d group and14d group. A, B,C,D,E,a,b,c,d,e ten group (model group to uppercase letters,control group to lowercase letters),6rats in each group, normal feeding.High-frequency surgery for20W power condensate1s,Taking each point bile duct to do the HE dyeing and CTGF immunohistochemical stains and carries on the observation and analysis.Results:By using two sample mean t test,analysis of modeling group blood sugar levels before and after comparison:male and female in the two groups before and after the drug had significant difference in blood sugar levels (P<0.05),so ALX has significant effects on diabetic rabbit blood glucose concentration change.Analysis of male and female in the two groups respectively forthe comparison of blood sugar levels before giving the medicine and after the treatment:male and female two groups before delivery has no obvious difference in blood sugar levels(P>0.05),there are significant differences in blood sugar levels after the treatment (P<0.05),so ALX is larger than the effect on female rats blood glucose effects on male rabbit. Pathological changes of bile duct wall structure,1d group visible electric injury of bile duct wall tissue large gasification necrosis;gasification area wall structure damage,accompanied by a small amount of neutrophils and inflammatory cells infiltration;3d group visible electric injury of inflammatory necrosis,visible around the necrotic inflammatory granulation tissue and fibroblast proliferation,associated with inflammatory cells infiltration;the change characteristicsof bile duct electric injury for the5d are basically with the same as3d group, Accompanied by a fibrous tissue cell hyperplasia,bile duct wall thickening of the3d,In mononuclear cells and inflammatory cells infiltration;7d group electric coagulation necrosis changes in bile duct epithelial cells,accompanied by connective tissue hyperplasia,give priority with lymphocytes and plasma cells infiltration,the wall is thickening.The control group of the damage range of the average (4.05±0.38) mm. modeling group of the damage range of the average (4.03±0.37) mm.Analysis of variance for repeated measurement data,Between the main body effect of inspection findings:modeling group and control group there was no significant difference to the influence of CTGF significance (P=0.312).Mauchly spherical test statistics W=0.012 (p=0.000),So refused to spherical,the univariate analysis to needs epsilon correction.Within the body effect of inspection found that:significant difference between different time group (p<0.01),But the different time and modeling group and control group without interaction (p>0.05).After pairing comparison found:except to the5d and7d group had no significant difference in outside (P=0.055,P=0.055),the rest of the differences between groups in different time had significant difference (P<0.05).Conclusion:The rabbit ear margin vein intravenous injection of alloxan induced New Zealand white rabbit effectively form of type1diabetes,female than male rabbit rabbit is more suitable for type1diabetes induced by alloxan model was constructed. Scope of extrahepatic bile duct injury of electric bright actual damage is greater than the naked eye can see. High blood sugar condition of extrahepatic bile duct electric burns of gasification necrosis, inflammatory pathology to repair the tissue cells necrosis, coagulation necrosis, hyperplasia of fibrous tissue, the development of the tube wall fibrous thickening, resulting in the happening of the bile duct stenosis. Compared with normal blood sugar, high blood sugar condition to a certain extent, slow down the process of the extrahepatic bile duct stricture, but also increased the probability of bile leakage occurred. The expression of CTGF can obviously found in hyperglycemia state electric injury early bile duct hyperplasia of fibrosis in the process of repairing weaker than normal blood sugar condition.