| Liquid core waveguide (LCW) have the function to confine the light signal in a special path based on the inner total reflection. When liquid core waveguide is used in CL analysis, we can detect light signal in the end of LCW. Due to the small detecting window, liquid core waveguide technology can be used for array detection in the future. In this paper, a chemiluminescent immunoassay system was developed utilizing Teflon AF-coated quartz capillary and sequential injection system, which features simple structure, low consumption, fast analysis and convenient operation. The system was used for the detection of tumor marker a-fetoprotein (AFP).In chapter1, the basic knowledge, methods of immune analysis and the characteristics of microfluidic immune analysis were reviewed, and also the application of chemiluminescence immune analysis in tumor marker detection was introduced. The liquid-core waveguide technique was introduced, and its application in chemiluminescence detection was also summarized. Finally, the purpose and design of this work were proposed.In chapter2, a chemiluminescence immunoassay system based on Teflon AF-coated quartz capillary was established, and it was used for the detection of tumor marker AFP. The AFP antibody was immobilized at inwall of the Teflon AF-coated quartz capillary using a covalent bonding method, and sample volume and flow rate were controlled by a sequential injection system. Various key parameters governing the analytical performance of the system were investigated, including incubation time, substrate volume, substrate flow rate, volume fraction of HRP-labeled anti-AFP. Linear range of this approach was5.0-800ng/mL with LOD of0.52ng/mL (S/N=3) and a precision of7.1%RSD (n=3). This method was applied to the determination of AFP in serum from suspected liver cancer patients, and the recoveries were95.4 -104.3%In chapter3, the chemiluminescence immunoassay system for detection of AFP based on LCW was summarized. |
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