Antitumor Constituents From The Fruiting Body Of Phellinus Igniarius Ethanolic Extrac

The fruiting body of Phellinus igniarius Quelet has been used in oriental countries for treatment of various diseases including cancer. However, it is unclear which kind of chemical compounds from the fruiting body of P. igniarius exerts anticancer effects. Activity-guided fractionation based on cytotoxcicity lead to isolation of eight compounds (Ⅰ-Ⅷ) from the dichloromethane fraction of the fruiting body of P. igniarius ethanolic extract. Their structures were identified as3-acetyl oleanolic(Ⅰ)、 lupeol(Ⅱ)、3-hydroxy methylation-4α,14α,20R-triol-9,19-cyclo-24(31)-en-5α--cholestane(Ⅲ)、23-hydroxy betulinic acid(Ⅳ)、β-sitosterol(Ⅴ)、 betulin(Ⅵ)、 olean-13(18)-en-29-oic acid,3-hydroxy-(3β,20α)(Ⅶ)、lup-1β,3β,11α-triol(Ⅷ) by spectral evidences. In cytotoxic assays in vitro, compounds Ⅰ、Ⅱ、Ⅳ、Ⅵ and Ⅶ exhibited strong cytotoxicities with IC50values of6.6、20.0、35.9、9.7、21.4μM, respectively, against human epithelial carcinoma cell line (HeLa cells), and compounds Ⅰ、Ⅵ、Ⅶ、and Ⅷ exhibited strong cytotoxicities with IC50values of9.9、23.5、9.8、32.4μM, respectively, against human hepatocellular liver carcinoma cell line (Hep G2cells).