The Study On The Effects Of Immunoregulation To The Immunocompromised BALB/C Mice With Traditional Xinjiang Fermented Camel Milk

Objective: To use Cyclophosphamide (CTX) to induce normal mice’simmunosupression for the establishments of models[7], and they were compared andmeasured with those of the high dosage group, medium dosage group and low dosagegroup of fermented camel milk ad explored the coordination and enhancement capacitiesof the fermented camel milk. Method: Through the study of the effects of fermentedcamel milk on the immunity of mice, MTT assay was used to detect the killing activitiesof NK cells, flow cytometry was used to detect the percentages of the T-cell sub-groups ofCD3+, CD4+, CD8+specific antibodies, and ELISA was used to detect serum cytokinesfor the detection of IL-23，IL-27and IFN-γ. Result:1. compared with the normal group,the thymus and spleen weights and their coefficients of the model group were markedlyreduced, and the percentages of the CD3+, CD4+and CD8+cell sub-groups of the mice’sspleens were markedly reduced.2. In the comparison of the model group and thefermented camel milk group (0.01，0.02and0.04g·kg-1), among them it can be seen thatthe changes of the0.02g·kg-1thymus and spleen weights and their coefficients were moreobvious.3. In the comparison of the model group detected with the MTT assay and thefermented group, in the fermented camel milk group, the killing activities of the NK cellsin mice were obviously enhanced, with the killing rates being respectively （39.8±0.6**##）（37.4±0.8**）（37.5±0.7**）and (38.8±0.6##）.4The flow cytometry was used for thedetection of T-cell sub-groups, and in the comparison of the model group and thefermented camel mil group, the percentages of the mice’ peripheral blood CD4+、CD8+of the model group were markedly increased; the fermented camel group (0.01，0.02and0.04g·kg-1) could reduce the percentages of the mice’s peripheral blood CD4+and CD8+cells(P<0．01或P<0．05) treated with CTX to various extents.5. ELISA was used was used forthe detection of serum cytokines of IL-23，IL-27and IFN-γ, and in the comparison of themodel group and the fermented camel milk group (0.01，0.02和0.04g·kg-1),the cytokinesof the medication group were all higher than the fermented milk group.Conclusion: thethree medication groups which were given the fermented camel milk all exerted effects onthe thymus and spleen weights and their coefficients, the killing activities of the NK cellselevated, and they could increase the sub-group percentages of the mice’s CD3+、CD4+、CD8+cells; fermented camel milk featured enhancements on the mice’s non-specificimmunity and specific cellular immune response, and its mechanism might have been theelevation of the ratios of CD4＋/CD8＋by elevating the sub-group percentage of theCD4＋、CD8＋T lymphocytes, especially the cell count of CD4, thus increasing the ratiosof CD4＋/CD8, activating and elevating the lymphocytes with immunological effects andfurther playing the role of immunological enhancement. Fermented camel milk could themice’ versera indexes with low immunity, improve the low spleen lymphocyteproliferation reaction in mice with immunosuppression, increase the counts of CD4＋、CD8＋cells of the mice’ peripheral blood and elevate the levels of the cytokines; thetraditional fermented camelmilk featured certain regulating functions on the mice’simmunological function caused by CTX.