Relationship Between The Promoter Methylation Of IFITM1, HS3ST2Genes And The Cervical Cancer In Xinjiang Uigur Women

Objective： To explore the relationship between the gene promoter methylation of IFITM1, HS3ST2genesand the cervical cancer of Xinjiang Uygur women and the feasibility of the early detection. To analyze themRNA expression level of IFITM1, HS3ST2genes in methylatiion-positive and methylatiion-negativetissues and detect the protein expression level of IFITM1in the cervical cancer tissues and normal cervixof Xinjiang Uygur women.Methods： Methylation specific PCR (MSP) and HPV16, HPV18specific PCR were used to detect theIFITM1gene promoter methylation and HPV16, HPV18infection in40normal cervix and40cervicalcancer tissues of Xinjiang Uygur women, to detect the HS3ST2gene promoter methylation and HPV16,HPV18infection in40normal cervix,10cervical intra-epithelial neoplasia (CIN I),10CIN2,10CIN3and40cervical cancer tissues of Xinjiang Uygur women. The relationship between IFITM1, HS3ST2genespromoter methylation and HPV16, HPV18infection was also investigated. RT-PCR was used to analyzethe mRNA expression IFITM1, HS3ST2genes in10IFITM1, HS3ST2genes promotermethylation-positive and10methylation-negative cervical tissues. The IFITM1protein expression levelwas analyzed in35normal cervical tissues and35cervical cancer tissues of Xinjiang Uygur women byimmunohistochemistry.Results：The methylation frequency of IFITM1gene was31/40and3/40in cervical cancer and normalcervical tissue samples. None of the40normal cervix samples and the10CIN1tissue samples weremethylated, however,5of the10CIN2samples,7of the10CIN3samples and38of the40cervical cancertissues were methylated, giving a methylation rate of50%,70%and92.5%respectively.The correspondinginfection frequency of HPV16/18is5/40,1/10,3/10,5/10and27/40. or the IFITM1and HS3ST2genes showed higher methylation frequencies in HPV16/18positive patients compared with in HPV16/18negative patients in40cervical cancer tissues and60CIN2or higher cervical tissues, respectively (bothP<0.05). The mRNA expression level of IFITM1and HS3ST2genes was more decreased in10methylation-positive cervical tissues than in methylation-negative cervical tissues (both P<0.01). Theprotein expression of IFITM1gene was also lower in35normal cervical tissues than in35cervical cancertissues（P<0.01）Conclusion： The promoter metylation of IFITM1gene decreased the gene expression, the IFITM1genewas the cancer suppressor gene in Xinjiang Uygur women. The promoter methylation of HS3ST2gene decreased the mRNA expression in cervical cancer tissues. HPV16/18affected the promoter metylationof IFITM1and HS3ST2genes and the hypermethylated HS3ST2may be the critical marker for thecervical lesion that will progress to cervical cancer and has the potential for the early detection of cervicalcancer.