| Objective: To observe the effects of p-JNK expression of dorsal root ganglia and pain behavior in ratswith CCI of different doses of pregabalin.Methods：60female SD rats were randomly divided into5groups,12rats for each group, includingsham-operation group (group S),CCI group (group c), group P1, group P2and group P3which involvingdifferent doses of pregabalin. In group S, the sciatic nerve was exposed but not ligated, and in group C andgroup P1, P2and P3, chronic constriction injury was established.7mg/kg,14mg/kg and30mg/kg ofpregabalin were injected separately and intragastricly into group P1, P2and P3in each morning andevening at postoperative1day, and physiological saline of the same volume was injected separately andintragastricly into group S and group C. Paw withdrawl latency (PWTL)was detected in each group at1d before operation and postoperative3,7,15day. After detection of PWTL at postoperative3,7,15day,four rats were randomly selected from each group and detected p-JNK expression of dorsal root ganglionwith immunohistochemical technique.Results:(1) The results of PWTL: compared with group S, the results of PWTL in group C and P1atpostoperative1d,3d,7d,15d was obviously declined (P<0.05), and in group P2, the results of PWTL atpostoperative1d,3d,7d was obviously declined(P<0.05), there was no statistically significant differencein the results of PWTL in group P3at postoperative1d,3d,7d,15d(P>0.05); compared with the result at1d before surgery, the results of PWTL in group P1at postoperative1d,3d,7d,15d was obviouslydeclined (P<0.05) and the results of PWTL of group P2at postoperative1d,3d,7d and of group P3atpostoperative1d,3d,15d were declined (P<0.05)；compared with group C, the results of PWTL of P1atpostoperative3d,7d,15d were obviously increased (P<0.05) and of group P2and group P3atpostoperative1d,3d,7d,15d was obviously increased (P<0.05); compared with group P1, the results ofPWTL at postoperative1d,3d,7d,15d in group P2and P3was obviously increased (P<0.05). Comparedwith group P2, the results of PWTL of P3at postoperative1d,3d,7d were obviously increased (P<0.05).(2) Immunohistochemical score of p-JNK: compared with group S, the score of p-JNK in group C andgroup P1at postoperative3d,7d,15d was increased, and in group P2, the score at postoperative3d,7d wasincreased (P<0.05), there was no statistically significant difference in the score of group P3atpostoperative3d,7d,15d(P>0.05)；compared with group C, the score of p-JNK in group P1, P2and P3atpostoperative3d,7d,15d was obviously declined(P<0.05)；compared with P1, the score of p-JNK ingroup P2and group P3was obviously declined at postoperative3d,7d,15d (P<0.05)；compared withP2, the score of p-JNK in group P3was obviously declined at postoperative3d,7d (P<0.05).Conclusion: The p-JNK expression in the side of surgery of dorsal root ganglion of chronic constrictioninjury rats was obviously increased, pregabalin of different doses shows an analgesic effect on rats withneuropathic pain caused by chronic constriction injury, which is possibly associated with inhibition ofactivation of p-JNK, with dose-dependent characteristic. |
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