Study On Inhibiting Effect Of New Recombinant Toxin On Angiogenesis And Growth Of Ovarian Cancer

Objective: To study the effect of new recombinant toxin uPAa-melittin inthe growth and angiogenesis of ovarian cancer.methods: Hmuan ovarian cancer transplanted subcutaneously in nude micewas established and randomly divided into5groups,each group contains7nudemice.(1) Low dose group of uPAa-melittin:0.25mg/kg.(2) middle dose group ofuPAa-melittin:0.5mg/kg.(3) High dose group of uPAa-melittin:1.0mg/kg.(4)DDP group:2mg/kg,0.2ml.(5) control group: soline solution. Each groupaccepts the dose of0.2ml intraperitoneal injection. Five different ways oftreatments were served from day8after transplantation and all nude mice weresacrificed after21days, three times a week, a total of6injection. After the startof the experiment, observe the general state of nude mice every day, includingthe diet and drinking and the response to external stimuli, to observe the growthof subcutaneous transplanted tumor, at the same time, use the vernier caliper toobserve the tumor diameter and short diameter, to calculate the tumor volumeand calculate the tumor inhibition rate of each group. The VEGF(vascularendothelial growth factor) and the MVD (tumor micro vessel density) weredetected by the immunohistochemisty in tumors tissue. Results:(1)The growth of tumor in low dose group of uPAa-melittin,middle dose group of uPAa-melittin, high dose group of uPAa-melittin and DDPgroup was significantly reduced compared with the control group. Inhibition rate30.36%,30.85%,38.99%,60.59%.(P<0.05).(2) control group MVD9.7±1.0, the group of uPAa-melittin MVD6.5±0.6, DDP group7.4±1.5(P<0.05).Conclusions1. DDP group and the Low dose group、middle dose group、high dosegroup of uPAa-melittin can significantly inhibit the growth of human ovariancancer transplanted subcutaneously in nude mice compare with the controlgroup. The difference was statistically significant. With the increase ofuPAa-melittin dose, the inhibition of tumor growth is more obvious, showing acertain dose effect relationship. At the same time, compare the inhibitory rate oftumor of each group can also support the above view. Compare the weight ofnude mice in each group during the experimental period, the mice weight inDDP group decreased significantly compared with the control group and theuPAa-melittin group, the difference was statistically significant. The weight ofnude mice in uPAa-melittin group compared with the control group express nostatistical significance, thus proving that the toxicity of uPAa-melittin isrelatively small, relatively safe.2.The positive expression rate of uPAa-melittin VEGF was significantlylower than that of control group (P<0.05), indicating that uPAa-melittin can inhibit the expression of VEGF in ovarian cancer tissue. At the same time, thecontrol group, the microvessel density (MVD) was higher than that inuPAa-melittin group, the difference was significant.(P<0.05). UPAa-melittincan reduce the MVD in human ovarian cancer transplanted subcutaneously innude mice, showing the effect of anti angiogenesis in tumor.