| Tooth development is a long, complex process of continuous occurrence,including tooth and tooth formation (morphogenesis), cell differentiation(differentiation), tooth eruption (eruption) and several stages. Tooth enamel knotsappear in the cap stage and bell stage and disappear in the late tooth development astemporary structures. Located in central tooth enamel knot, studies show that theenamel knot is to adjust the signal center in early tooth morphogenesis. Therefore, thisexperiment will explore the important role of enamel knots in tooth development.Weuse the mouse tooth germ epithelial and mesenchymal recombination andtransplantation to confirmed enamel knots play important role in the formation oftooth size and number of teeth tips. Followed by situ hybridization method to track thelocation of the enamel knot, we found that even remove enamel junction epitheliumpart in the subsequent process of development will grow new enamel junction. Theresults tell us that the enamel knot indeed has important role in tooth development andis an essential part.Due to the lack of suitable seed cells in clinical treatment of cartilage defects, ithas not been established fully effective technical methods to the rescue. Therefore, thedevelopment of new cartilage tissue engineering seed cells has important scientificsignificance. Stem cell technology is today’s most cutting-edge field of regenerativemedicine. We establish a system in vitro differentiation of cartilage from stem cells.This method solves the problem of seed cartilage cell research, but also provides agood foundation platform for cartilage repair. This experimental method is throughmaking EBs to induce cartilage differentiation in vitro. We successfully induced tissuesamples with cartilage features, namely cartilage. And we have provided some basisfor the development and repair of cartilage disease to further research of cartilage. |
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