Generation Of Microgels On Microfluidic Chip And Its Application

Myocardial infarction poses a serious threat on survival and life quality of human. As a new treatment, encapsulation of cells in microgels developed rapidly in recent years. Conventional methods used for the encapsulation of cells in microgels include bulk emulsification, electrostatic dripping, extrusion methods and hydrodynamic dripping. These methods have a number of limitations, including a broad distribution of microgel size, large microsphere dimensions and high consumption of reagents.As a promising discipline, microfluidic has a wide range of applications in chemistry, biology and medicine. Generating microgels in microfluidic devices has many advantages. It consumes less reagents. It is simple to operate. It has a higher monodisperse coefficient, and it may generate microgels with high throughput.In this thesis, we designed a T-type channel to genetate droplets. After geling of the droplets, we could get microgels. In the process of geling, we didn’t need light or initiators. Therefore, we avoided the damage on cells in the geling process. We investigated the effects of GCS concentrations, span80concentrations on the formation of droplets. Besides, we could control the size of the droplets by varying the flow rates of the continuous phase and the dispersed phase.Subsequently, we evaluated the biocompatibility of the hydrogel with H9C2and CGR8cells. MTT assay proved DF-PEG had good biocompatibility, and DF-PEG had no effect on NOS activity in H9C2cells and ROS activity in CGR8cells. After cultured in3D hydrogel, the survival rates of both cells were above70%in72h. The morphology of the cells is much closer to cells in vivo. At last, we encapsulated the cells in microgels for the treatment of myocardial infarction in the future.