Study On Quality Standard Of Tibetan Drug Bangna

Bangna, a Tibetan medicine is commonly used by folk and clinical doctor for its anti-inflammatory, analgesic and anesthetic effects, primarily for the treatment of lumbar muscle strain, rheumatoid arthritis and other diseases of this kind. Bangna is the dried root of Genus Aconite plants of Buttercup that contains alkaloids of which Aconitum Diester Diterpenoid Alkaloids are toxic components. It has important clini-cal significance to study the quality standards of Bangna as alkaloids contained in Bangna are the active ingredients and toxic components.The quality standard of Bangna is not contained in the Chinese Pharmacopoeia (2010), and in the Ministry of Health standards·Tibetan medicine (1995) is only part of the simple provisions and very imperfect, it just contains [Description],[Identifica-tion],[Action],[Indications],[Usage and dosage] and [Storage] provisions and is lack of [Content] and [Check] provisions of active or toxic ingredients which are as signs of modern pharmaceutical quality standards. a comprehensive quality standard system needs to be establish to effectively control the safety and efficacy of the drug.This study carried out the method for determination of water, total ash and extract in the Chinese Pharmacopoeia (2010) to measure the Bangna, and tentative the limits were12.5%,7%and15%, also established aconitine limited assay method by HPLC which is specific, durability, high precision. The average recovery was99.15%, RSD=0.7%. Using UV-visible spectrophotometry, the establishment of a method for the determination of total alkaloids, applied to the determination of good reproducibility, RSD=2.54%, the average recovery was95.41%, RSD=0.43%. Besides, we also studied TLC method of Bangna, but it has not mutual spots.