| Rice bran is the main by-product produced by paddy rice（Oryza sativa）,belongs to Gramineae, Oryza. Paddy rice widely grown in East Asia, South Asia,is an important food crops. The current study mainly involves rice bran oil, ricebran, phytic acid and inositol, etc, While relatively few aspects of the study of thepolysaccharide. Therefore, In this study, rice bran as raw material, its extractionprocess of polysaccharides, immunomodulatory activity and buccal tabletpreparation process was studied.The main results were as follows:（1）Rice bran alkaline-soluble polysaccharides were extracted from the cellwall of the Rice bran by aqueous alkali extraction method, and precipitated with5times vo-lume of95%ethanol. In order to increase the yield of rice branpolysaccharides, extr-action process was optimized by the orthogonalexperimental design method on the basis of the results of the single-factor tests.The results showed that affect the yield of rice bran SPK-1from primary andsecondary order as follows: the concentration of pot-assium hydroxidesolution(D)>extraction temperature(B)>extraction time(A)>liquid ratio (C).Theoptimal extraction condition for the yield of rice bran polysaccha-ride were asfollows: extraction temperature was50℃; extraction time was3hrs; liquid-solidratio was25:1and the concentration of potassium hydroxide solution was2mol∕L.The rice bran polysaccharide rate was2.69%, with the theoreticalprediction2.71%were similar, suggesting the best extraction method of theorthogonal optimization of rice bran polysaccharide is feasible.At the same time,the measurement of the content of polysaccharide of rice bran polysaccharideSPK-1is87.04%, SPK-2is94.68%，the pentose content of SPK-2is54.05%.（2）Experimental studies of rice bran polysaccharide immune activityshowed that:when using CCK-8assay rice bran samples of mouse spleenlymphocytes in vitro activity of the role of impact, found250μg/ml,50μg/ml,10μg/ml,2μg/ml concentra-tion, SPK-1can significantly promote the proliferation of mouse spleen lymphocytes, SPK-2when250μg/ml concentrationcould significantly promote the proliferation of mouse spleen lymphocytes andwithin the range of concentrations tested did not show a significant toxic effects.When the sample is detected on cytokine secretion by ELISA observed rice branSPK-1at25μg/ml,5μg/ml two concentrations, can signific-antly promoteConA-induced mouse spleen lymphocytes secrete IFN-γ. The SPK-2at5μg/ml,1μg/ml can significantly promote the secretion of IL-2; at25μg/ml, when5μg/mlcan significantly promote the secretion of IFN-γ in; at5μg/ml, suppressed when1μg/ml secretion of IL-10;1μg/ml when IL-17can inhibit secretion.（3）To study the preparation process of rice bran polysaccharide buccaltablet, preliminarily determine the buccal tablet preparation process as follows:Rice bran20g, lactose20g, mannitol10g, CMS-Na1g, wetting agent choose70%ethanol aqueous solution, magnesium stearate0.45g, citric acid0.20g. Accordingto the prescription system control in grain drying grain moisture from1.1%to4.1%, the particles of liquidity, good formability are and ethanol residue andbrittle broken conform to requirements.Rice bran polysaccharide SPK-2powder and sugar of accessories, mannitol,with70%ethanol wet granule,55℃drying,20mesh granulation, add citric acidtablet and a moderate amount of magnesium stearate. Through the process ofbuccal tablet partial bright and beautiful, disintegration degree is better, show thatthe technology is feasible.Through the determination of buccal tablet weightvariation,disintegration and dissolution test, provide experimental basis for furtherstudy on the rice bran polysaccharide buccal tablet. |
PDF Full Text Request