Effect Of Oral Treatment With Lysed Enterococcus Faecalis FK-23(LFK) On Expression Of CD80and CD86in A Murine Model Of Allergic Rhinitis

Background:Although it is widely recognized that the intake of so-called probiotic microorganisms is beneficial in chronic mucosal inflammation and topical allergic disease, the immunologic details explaining how such bacteria can exert these effects remain obscure.Objective:To We determined whether lysed Enterococcus faecalis FK-23(LFK) modulate the expression of costimulatory molecules CD80and CD86in the spleen of murine allergic rhinitis model.Thl/Th2balance responses in vivo.Methods:BALB/c mice were intraperitoneally injected with ovalbumin (OVA) and challenged intranasally with OVA. The LFK preparation were administered orally daily for3weeks, respectively. Meanwhile, OVA-sensitized group (OVA group) and non-sensitized control group (control group) which administrated orally with saline were set up. The costimulatory molecules CD80and CD86expression in the spleen were measured by immunohistochemistry. The levels of the Thl cytokines IL-12were quantified in serum using ELISA.Results:Serum IL-12levels significantly decreased in LFK-treated group compared to the OVA group. The immunohistochemical analysis results showed an down-regulation of CD80after the last OVA challenge, and it was significantly activated by LFK treatment. Otherwise, CD86was showed decrease in the LFK-treated group compared to OVA group mice.Conclusion:These results indicate that the oral administration of LFK may alleviate the nasal symptoms in murine allergic rhinitis model. LFK may modulate the expression of costimulatory molecules CD80and CD86in the spleen, and change the levels of IL-12in serum. LFK may change the expression of costimulatory molecules CD80and CD86in the spleen. These data suggested that the probiotic prepartions have the action of anti-allergy and immunomodulation.