| Objectives: To study the effect of heliox-driven nibulization in differentconcentration proportion of oxygen on COPD rats by measuring lung function, the level ofIL-6in serum,the expression level of HIF-1α factor in lung tissue and observing changesof pathological section microstructure to judge the treatment results of heliox-drivennebulization, provide a reliable scientific evidence for COPD patients using heliox-drivennebulization.Methods: Establishing34rats models of COPD using the exposure to cigarette smokeand intratracheal injection of lipopolysaccharide(LPS), with the control group12, wen themodels are established, the model group and the control group were randomly take six toverify whether the model is successfully established. After COPD models were successful,the remaining28rats were anesthetized COPD femoral arterial blood gas analysis,according to the arterial oxygen saturation SaO2values and the method of random blockrats were randomly divided into four groups, the control group was6, the five groups offive rats were housed in the same cage size model, inhalation into three groups, the threedrivd gas have medical oxygen gas (equivalent oxygen fraction of45%), Heliox (He71%:O229%), Heliox (He63%: O237%), the fourth group of the model control group and thefifth group control group wirhout treatment. The labels cover cylinder gases, paste A, B,C ’s label, blinded to achieve the purpose. Inhalation method2times/day, every15min,gas flow6L/min, sustained two weeks., atomized liquid of0.9%saline5ml,chymotrypsin04,000units, inhalation Pulmicort (budesonide)2ml and Atrovent(inhalation ipratropium bromide solution)2ml, Atomization process closely observed inrats, pay attention to their behavior.After two weeks of nebulization, extracted rat femoral arterial blood gas analysis,and then the rats were connected AniRes animal lung function analysis system on rat lungfunction. After testing, blood was collected from the abdominal aorta5ml,4000r/min,centrifugal radius of20cm, centrifugal10min, the serum stored at-20℃to detect theexpression levels of IL-6. Exposure to the chest, through the ascending aorta cannulation,fast perfusion of saline150-200ml, right atrium notches until the effluent colorless; thenthe left and right lung tissues were collected, frozen-80degrees spare. Production biopsyto detect hypoxia inducible factor HIF-1α factor expression levels. Results Experimental group compared with the control group, ODN groupinspiratory resistance Ri,,there is no significant difference in lung dynamic complianceCydn with the control group (P>0.05), two groups with HDN compared to model group,Ri significantly reduced, Cydn significantly increased, and the difference was statisticallysignificant (P <0.05). ODN group and HDN (He71%: O229%) group compared with themodel group expiratory resistance (Re) was significantly lower, the difference wasstatistically significant (P <0.05). HDN group (He63%: O237%) compared with the modelgroup, the difference was not statistically significant.Each index difference ANOVA five groups of rats before and after atomization resultsshown, PaCO2, PH, SaO2difference was statistically significant, PaO2difference was notstatistically significant. Five groups of rats before and after each index atomizationdifferences using paired t test was used for analysis, the results are shown. PaO2valuesbefore and after nebulization no statistically significant difference between groups. PaCO2values ODN and HDN (He63%: O237%) group difference was statistically significant, theatomization PaCO2significantly higher than before atomization; fog before other groupsshowed no significant difference. PH value of the ODN and HDN (He63%: O237%)before nebulization group difference was statistically significant (p <0.05). SaO2valuesHDN group (He71%: O229%) was statistically significant difference before and afteratomization, atomized before other groups showed no significant difference.HDN (He71%: O229%) comparing with the model group, IL-6expression levelswere significantly lower, the difference was statistically significant (p <0.05); HDN(He71%: O229%) group and ODN group compared with the model group, the differencewas not statistically significant (p>0.05).Quantify the expression of HIF-1α ODN group was22203.53±8285.79, HDN(He71%: O229%) group to quantify the expression of HIF-1α was27429.63±10811.39,HDN (He71%: O229%) group to quantify the expression of HIF-1α was17292.36±7951.34, model group, HIF-1α expression quantified as24689.38±7738.49, theexpression of HIF-1α control group to quantify19505.39±4055.16, single-factor analysisof variance between groups displayed significant difference (p=0.007), pairwisecomparison, HDN (He71%: O229%) group compared with model group, the expressionlevel was significantly lower, the difference was statistically significant (p=0.014), otherthan the group with the model group, the level of expression of the difference was notstatistically significant (p>0.05). Conclusion: HDN (He71%: O229%) and HDN group (He63%: O237%), comparedwith medical oxygen, Heliox (He71%: O229%) can reduce the resistance Ri and Re,improve Cydn, reduce PaCO2increase PH. And HDN (He71%: O229%) group COPDlung tissue expression of HIF-1α lowest level, lower serum IL-6cytokine levels, heexplains the symptoms of COPD hypoxic rats eased, on the other hand description of thedrug to reach the distal bronchial, played a role in anti-inflammatory, to the effect ofatomization.The effect of HDN (He71%: O229%) is better, both the drug delivery terminalbronchioles, to atomizing effect, but also the distal bronchial and alveolar oxygen deliveryto ease the symptoms of COPD hypoxia.It can provide reliable scientific basis for clinicaltrials or clinical applications late. |
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