Macrophage Migration Inhibitory Factor And Phospholipid Transfer Protein In The Related Research Of Bronchial Pulmonary Dysplasia

Objective:Collection premature serum and bronchial lung lavage fluid, comparing BPDpremature infants and the control group at different time points and bronchial lung lavagein serum MIF and the variation in content of PLTP, explore MIF and PLTP in BPDpremature serum level and relation with BPD disease development, for the early diagnosisand prognosis of BPD judgment to provide laboratory basis; Analysis the clinical data ofchildren with BPD, have disease cause of bronchial pulmonary dysplasia (BPD), clinicalcharacteristics, disease diagnosis, and treatment, and outcome, to prevent the risk factors inadvance and provide reliable basis for early diagnosis of diseases, and provide new train ofthought for its treatment and prevention.Methods:1Using radial artery puncture method were within24hours after birth in pretermchildren, one week, two weeks, three weeks, four weeks specimens were taken. Connectedto5ml with scalp acupuncture needles, left hand holding the wrist so that preterm childrenpreterm children wrist extensor Yang approximately45°, touch the radial arteryfluctuations, skin disinfection, right hand holding the needle, in the vicinity of the radialartery in the wrist stripes parallel to the horizontal direction was30°~45°angle piercing,blood drawn about2ml,4°,3000r/min, centrifuged10min, the supernatant was placedspecimens stored for use within-80℃refrigerator.2Bronchoalveolar lavage fluid collected using non-bronchoscopic bronchoalveolarlavage fiber Act (bronchoalveolar lavage, BAL), neonatal mechanical ventilation orendotracheal intubation tracheal tube after sedation after instillation of sterile saline37per0.5mL/kg, dripping after use pressurized oxygen resuscitator2-3times turning shot behindthe suction tube is inserted along the endotracheal tube to slightly tucked up quickly after encountering resistance, with less than6kPa suction, suction tube side twisting action toattract side exit endotracheal intubation, the whole operation is completed in the30s,bilateral3times each. Recycling was centrifuged (4,500r/min)10min, the supernatant wasplaced in-80refrigerator spare, also within24hours after birth in preterm children, oneweek, two weeks, three weeks, four weeks specimens were taken.3The central hospital of Tai’an City was retrospectively analyzed in25cases ofpatients with BPD details, the gestational age, birth weight, primary disease, and lung CRradiological findings, treatment and outcome of clinical data for analysis.Results：1In the serum samples, the experimental group compared with the control group,serum MIF levels at time points24hours, one week, two weeks, three weeks, four weekswere higher, the difference was statistically significant (P〈0.05), the experimental groupMIF levels remained high, with relatively low levels in the control group; levels of serumlevels of PLTP in24hours, one week was not significantly different (P〉0.05), at2weeks,3weeks,4weeks, BPD group than the control group, the difference was statisticallysignificant (P 〈0.01); while the experimental group at4weeks, the first week of thecontent of PLTP levels close to the control group (Table8).2In bronchoalveolar lavage fluid samples, the experimental group at each time pointMIF content levels were higher, the difference was statistically significant (P〈0.01),consistent with the serum results (see Table9); experimental group PLTP content werelower than the control group, the difference was statistically significant (P〈0.01), while theexperimental group and the control group at4weeks1week values similar (Table10).3A retrospective analysis of the clinical data of25cases of BPD, its gestational age,birth weight, primary disease, lung imaging, clinical data and outcome after therapy wereanalyzed.25cases in children with BPD, gestational age 〈28weeks in2cases,13casesof28~30weeks,30~33weeks of9cases,33weeks or more in1case; Birth weight〈l000g3cases,12cases of1000~1500g,1500~2500g8cases,〉2500g in2cases.BPD primary disease in10cases of neonatal respiratory distress syndrome (NRDS),inhalation syndrome6cases,7cases of pneumonia, pulmonary hemorrhage in1case,1case of normal. All children by oxygen treatment, the need for oxygen (34.6+10.9) d,16cases of mechanical ventilation, the need for mechanical ventilation (12+/-5.4) d, length ofhospital stay (62.0+13.6) d.19cases complicated with lung infection. Clinical cured anddischarged, the comprehensive treatments of13cases were7cases improved,5cases give up treatment automatic discharge.5cases are due to pulmonary infection readmissionwithin1year, and1case died.Conclusions1MIF and PLTP have high correlation with bronchial pulmonary dysplasia;2MIF of high level and low level of PLTP prompt premature BPD may exist, candetermine the level of BPD MIF and PLTP early diagnosis and is helpful to further activetreatment;3small gestational age, low birth weight are the important reasons for BPD, alsoaffect the risk factors of BPD dividing, first of all, prevent the happening of the pretermbirth in the clinical work, reduce the incidence of BPD;4premature immature lung development, mechanical ventilation, oxygen toxicity is acommon cause of BPD, secondary pulmonary infection in the process of treatment is topromote the development of BPD occur another important factor;5children with severe BPD GC use have certain effect, to take strong measures tocontrol secondary infections will help shorten the duration of BPD.