Comparative Study Of Antrochoanal Polyps And Ordinary Nasal Polyps’ Histopathology

Objective：Recently, a lot of studies about histopathologicalstructure of Nasal Polyps(NP) have reported.Tissue edema andinflammatory cell infiltration to be accepted by the public. As a special typeof Nasal Polyps, Antrochoanal Polyps(ACP) is different from NP inpredilection site and prognosis. These different clinical features may implythat the differences organizational structures between ACP and NP. Butthere are a few studies about the differences histopathological structurebetween ACP and ordinary NP. The purposes of this study were toinvestigate the pathological features of ACP and ordinary NP, to observe themucosal gland hyperplasia and the distribution of Eosinophils, Neutrophils,Plasmacyte and Lymphocytes in ACP and ordinary NP, meanwhile todiscuss the correlation between the inflammation cells and Microvesseldensity, to explore the differences histopathological structure between thetwo diseases, and the clinical significance. Methods: All the cases are takenfrom the Ya’an People’s Hospital Department of Otolaryngology betweenOctober2011to July2013which are choosed by the inclusion andexclusion criteria.24cases of ACP and40cases of ordinary NP werecollected.1. The submucosa gland hyperplasia of ACP group and ordinaryNP group were observed with Conventional HE staining, and the number of inflammatory cells(include Eosinophils, Neutrophils, Plasmacyte, Lym-phocyte) were counted under a light microscope.2. The expression ofCD34in the tissues were detected by immunohistochemistry EnVisionmethod. Results:1. Two cases of ACP had glands in thesubmucosa,incidence rate was8.33%,38cases of ordinary NP had glands inthe submucosa, incidence rate was95%, the data of the2groups wereanalyzed by chi-square test (χ2=48.071, P<0.05), the incidence rate ofglands in the submucosa in ordinary NP is statistically significant higherthan ACP group.2. The number of Eosinophils, Neutrophils, Plasmacyte,Lymphocytes and Microvessel density (MVD) in the ACP group and theordinary NP group were compared by independent samples t test, t valueswere18.423,9.564,4.823,2.100,2.930, P<0.05, a statistically significantdifference.3. In the ACP group, the Eosinophil cells and Neutrophils,Lymphocytes, Plasmacyte have positive correlation (r=0.943,0.751,0.630,P <0.01), the Microvessel density and Eosinophil, Neutrophils havepositive correlatoin(r=0.528,0.521,P <0.01).4. There was a negativecorrelation about the number of Eosinophils cells and Lymphocytes cells,Plasmacyte cells in the ordinary NP group（r=-0.686,-0.713, P<0.01）, thenumber of Eosinophil and Microvessel density have positive correlation(r=0.756, P<0.01). Conclusion:1. The incidence rate of glands in thesubmucosa in ACP group is statistically significant lower than the ordinarynasal polyps group, the types of infiltration cell in submucosal were different, the ordinary NP is mainly composed by Eosinophils, the ACP ismainly composed by neutrophils and lymphocytes. In the ordinary NPgroup.2. The proliferation of Microvessel is closely related to theoccurrence and development of nasal polyps. The MVD of ACP group wassignificantly lower than ordinary NP group. The degree of proliferationmaybe lead to the structure of ACP different from the ordinary NP.3.Through correlation analysis our study found that there was a positivecorrelation between the number of Eosinophil infiltration and microvascularproliferation with two groups of disease. It is suggested that eosinophils canpromote the proliferation of microvascular.