The Frequency Of HLA-A Alleles Of HIV-1Infected Former Plasma Donors In Central China And Its Association With HIV-1Infection

HIV is one of the members of the lentivirus families, which can directly target host immune system by infection of CD4+T lymphocytes, macrophages and dendritic cells. And the infection would be intereacted with the host immune responses, such as innate immune responses, humoral immune responses, and cell immune responses, which will result in different results in different individuals. As we know, individuals with equivalent levels of exposure show a differential propensity to become infected or demonstrate a differential rate of progression to severe immunodeficiency when infected. Most of the antiretrovirus-naive infectors progress to AIDS in6-10years (classic progressor), small subset of antiretrovirus-naive infectors can control replication of virus for more than15-20years which is called long-term non-progressor, including some elite controller who can control the virus level under the detection limit of currently available viral load assays, and there are also some infectors develop AIDS in2-3years of infection(rapid progressor). The manifestation of HTV-1infection is influenced by the characteristics of viral and host factors. Host genetic factors may have influence both on HIV transmission and disease progression. As reported, CTL plays an important role in controlling HIV acute infection and the strong CTL responses in acute HIV infection predict the lower virus set point, and CTL associated HLA alleles have been associated with effects on HIV infection and disease progression.HLA is the most polymorphic gene complex of the human genetic systems. The frequencies of HLA genotypes differ significantly among different human populations. Certain HLA genotypes showed influence on HIV infection and disease progression in patients from different risk groups with different ethnic background. The aim of our research is to study the distribution of HLA-A alleles in the former plasma donors(FPDs) from central China and further analyze the association between HLA-A alleles and HIV/AIDS status.47HLA-A alleles were detected in306participants with PCR-SSP and PCR-SBT techniques. The allele frequency ranged from0.001634to0.1339869. Of them, HLA-A*1101、2402、0201、0101、0301、3001,3303were among the most common alleles. When we compared the frequency between HIV-1infectors and healthy controls, we found that HLA-A*2402was significantly higher in84controls than in222infectors (P=0.01990.05OR=0.5446,95%CI0.3312-0.8955), which may suggest the possible association of HLA-A*2402and HIV-1infection resistance. Secondly, we investigated the association of HLA-A allele and clinical parameters shch as viral load (VL) and CD4T cell count in222infectors. We found that the VLs of individuals with HLA-A*0207、1101、3001alleles was significantly lower than individuals without these alleles, respectively (p value0.0234、0.0105、0.0138), which may suggest the negative association of these three alleles on viral fitness. While VL of individuals With HLA-A*2301allele was significantly higher than individuals without the allele(p value0.0097), which might present the positive association of the allele on viral fitness. Thirdly, we categoried the infectors with the level of VL and CD4T cell to investigate the association of HLA-A allele and the course of disease. We found that HLA-A*0101and2301alleles were associated with higher viral load and severe disease course.This study determined the distribution of HLA-A alleles in the FPDs from central China and explored the association between HLA-A alleles and HIV/AIDS status, the results obtained provide basic data on the chinese population polymorphic information, and may also shed light on HIV-1pathogenesis.