The Effect Of Lytic Cocktail And Hypothermia On Mice With Lipopolysaxxharide Induced Acute Lung Injury

Objective: To investigate the protect effect of lytic cocktail and hypothermia on mice with acute lung injury( ALI) induced by lipopolysaccharide( LPS).Methods: The mouse model of ALI was established by intraperitoneal injection of LPS(5mg/kg).Thirty-two male Sprague-Dawley rats,4-weeks of age, were randomly divided into 4 groups: nomal control group(NC group),lytic cocktail group,(L group), hypothermia group(H group), lytic cocktail+hypothermia(LH group).One hour after LPS injection,the lytic cocktail group(L group) and lytic cocktail+hypothermia(LH group)were inject lytic cocktail(Sabcutaneous injection of 12 m L/Kg lytic cocktail),the hypothermia group(H group)were inject saline(Sabcutaneous injection of 12 m L/Kg).At the same timesetting, the hypothermia group(H group) and the lytic cocktail+hypothermia(LH group) were feed in the cage holy padding with crused ice. Blood sample were obtained at 1hour、2hours、4 hours and 8 hours after LPS injection from each group. Plasma levels of interleukin-1(IL-1)、interleukin-10(IL-10)、Tumor Necrosis Factor-a(TNF-α)、myeloperoxidase( MPO) were determined with ELISA. Pathology changes in lung tissues were observed under light microscope with HE staining. Eight hours after LPS injection,the mice were sacrificed. The ratio of pulmonary wet weight to dry weight( W/D) was measured to assess the extent of pulmonary edema. Neutrophil infiltration was assayed by determining the interleukin-1(IL-1)、interleukin-10(IL-10)、Tumor Necrosis Factor-a(TNF-α)、myeloperoxidase( MPO) activity in plasma levels.Result:The lytic cocktail group(L group)、hypothermia group(H group)and lytic cocktail+hypothermia group(HL group)has lower W/D than the nomal control group(NC group, especially in lytic cocktail+hypothermia group(HL group). Pathology change coincidence with the W /D outcome. The results of the interleukin-1(IL-1)、TumorNecrosis Factor-a(TNF-α)、myeloperoxidase( MPO) activity of the nomal control group(NC group)increased over the time(P<0.05),but the interleukin-10(IL-10) has no statistical significance(P<0.05) over the time.The results of the interleukin-1(IL-1)、Tumor Necrosis Factor-a(TNF-α)、myeloperoxidase( MPO) activity of lytic cocktail group(L group)and the hypothermia group(H group) increased over the time(P<0.05),the interleukin-10(IL-10)decreased(P<0.05) over the time.The results of the interleukin-1(IL-1)、Tumor Necrosis Factor-a(TNF-α)、myeloperoxidase( MPO) activity of lytic cocktail+hypothermia group( HL group) decreased over the time( P < 0.05),the interleukin-10(IL-10)increased(P<0.05) over the time.Conclusion: Lytic cocktail may have protect effect on LPS induced ALI. Hypothermia may have protect effect on LPS induced ALI. The lytic cocktail and hypothermia may have a synergistic protect effect in this study by statistical analysis.