Rabbit Proximal Anterior Descending Coronary Artery Blood Nitric Oxide Dynamic Change And Study After Ligation

Objective: Dynamic observation rabbit before descending coronary artery ligation and oxidative stress electrocardiogram consistency factor, and to clarify the mechanism of its basic pathophysiological changes, and provide a theoretical basis for clinical diagnosis and treatment of myocardial infarction.Methods:42 healthy adult New Zealand white rabbits were randomly divided into A, B, C, D, E, F groups of six. By ligation of the left anterior descending coronary artery in rabbits, rabbits with acute myocardial infarction model produced, measured concentrations at different time points serum CK-MB, CTnT and NO. After ligation at different times through the left anterior descending coronary artery before Songzha 60 minutes, making rabbit model of myocardial ischemia and reperfusion, after Songzha detect serum concentrations of CK-MB, CTnT and NO for 60 minutes, and the whole record ECG change. A set of ligation 5 minutes Hou Songzha 60 minutes (n= 6), group B ligated 10 minutes Hou Songzha 60 minutes (n= 6), group C ligation 30 minutes Hou Songzha 60 minutes (n= 6), D group Hou Songzha ligation 1 hour 60 minutes (n= 6), E group Hou Songzha ligation three hours 60 minutes (n= 6), F group Hou Songzha ligation 6 hours 60 minutes (n= 6), and sham As a control group (n= 6). By ligation of the left anterior descending coronary artery in rabbits, rabbits with acute myocardial infarction model produced, measured concentrations at different time points serum CK-MB, CTnT and NO. After ligation at different times through the left anterior descending coronary artery before Songzha 60 minutes, making rabbit model of myocardial ischemia-reperfusion,60 minutes after the detection Songzha five minutes before descending coronary artery ligation in rabbits,10 minutes,30 minutes,1 hour,3 hours,6 hours,12 hours, after 24 hours and Songzha recorded before and after ligation of nitric oxide and ECG changes. Application Biovision nitric oxide assay kit (Colorimetric), rabbit creatine kinase (CK-MB) ELISA assay kit (Shanghai), rabbit troponin T (CTnT) ELISA assay kit (Shanghai). Each sample was measured by radioimmunoassay in plasma ET, CGRP concentration; the use Nanjing Jiancheng Bioengineering Institute of CK-MB, CTnT and NO kits, colorimetric assay for each sample plasma CK-MB, CTnT and NO concentration or viability. The data are expressed as mean ± standard deviation (i±s), said. Within groups and between groups were analyzed using one-way ANOVA, P<0.05 for the difference was significant.Results:1.CK-MB, among CTnT and NO group P<0.05, significant difference.2. The difference between the experimental groups within groups was statistically significant P<0.05.3 electrocardiogram:a rabbit model of acute myocardial infarction after ligation-reperfusion injury through arrhythmias have a higher incidence. With no ECG changes reperfusion injury group, reperfusion injury ECG changes of serum NO decreased significantly (P<0.01).Conclusions:1. Acute myocardial infarction, nitric oxide levels decreased significantly compared with before infarction. Trends:2 reperfusion after nitric oxide.2. Compared with enzymes, nitric oxide is not yet replaced as a new detection index.3. ST-segment elevation acute myocardial infarction after reperfusion therapy reperfusion injury prone ECG changes, which may be related to inflammation and aggravation increased production of oxygen free radicals after reperfusion related.