Antttumor Effect Research Of Arsenic Trioxide Nanoparticles On Breast Cancer Cell Line MCF-7 In Vitro

[Object] Breast cancer is one of common malignant tumor of female, which seriously affected the women’s quality of life and health. Arsenic trioxide as an important drug chemical auxiliary treatment of breast cancer, in recent years has been more and more attention by scholars and researchs. Nano drug delivery system because of its slow release, targeted, reducing the generation of tumor multidrug resistance and other advantages, is used as a kind of new drug application to the study of antitumor drugs. This experiment select arsenic trioxide nanoparticles as model drug.Explore the arsenic trioxide nanoparticles on human breast cancer MCF-7 cell cytotoxic effect, influence on cell cycle, induce cell apoptosis and the analysis the influence of human breast cancer cell MCF-7 cell of arsenic trioxide nanoparticles uptake. To explore with nanoparticles as a carrier of arsenic trioxide drugs for breast cancer in vitro antitumor effect of MCF-7 cell. To provide preliminary basis for the further experimental study and clinical application of this medicine.[Method] Determined by MTT method to measure the cytotoxic effect of human breast cancer which use different concentration of arsenic trioxide free drugs,blank nanoparticles, and arsenic trioxide nanoparticles;Using PI single dye flow cytometry detection of arsenic trioxide nanoparticles each cell cycle; Using Annexin V-FITC/PI double dye flow cytometry to detect apoptosis induced by arsenic trioxide nanoparticles; Using fluorescent microscope qualitative analysis the MCF-7 cell intaking arsenic trioxide nanoparticles; Using flow cytometry instrument to quantitative analysis the factors affecting the results of intake.[Results] After 24 h, free drugs to cell growth inhibition rate is greater than under the same concentration of arsenic trioxide nanoparticles drug; After 48 h, free drugs to cell growth inhibition rate is less than the same concentration of arsenic trioxide nanoparticles drug; When the concentration of drug is 1μg/mL and 3μg/mL, Arsenic trioxide nanoparticles effect after 24 h, Proportion of G0/G1 phase cells increases as the concentration increases, Proportion of S phase cells decreases as the concentration increases; Arsenic trioxide nanoparticles effect after 48 h, Proportion of G0/G1 phase cells increases as the concentration increases significantly, Proportion of S phase cells decreases as the concentration increases significantly; When the concentration of drug is 1μg/mL and 3μg/mL, Arsenic trioxide nanoparticles effect after 24 h, with the increacing of concentration, the rate of induce cell apoptosis increased; after 48 h, induction of apoptosis rate also increased with higher concentration, and were higher than under the same concentration effect after 24 h of apoptosis rate; Fluorescence microscope show MCF-7 cell of arsenic trioxide nanoparticles uptake occurred, Flow cytometry instrument quantitative display: With the increase of dosage cells for drug intake rate increase, With the increase of temperature, cells on drug intake rate increase, With the increase of duration, cells on drug intake rate increase.[Conclution] Drug carrier does not have cytotoxicity to the cells,do not produce inhibition for the growth of cells; rsenic trioxide nanoparticles have sustained-release effect, to the cytotoxicity and drug dosage, administration time were positively correlated; Arsenic trioxide nanoparticles by inducing cell cycle arrest in G0/G1 phase, play the role of the inhibition of cell proliferation; Arsenic trioxide nanoparticles in inducing cell apoptosis, cell apoptosis rate showed positively related to the dosage and duration of the relationship; MCF-7 cell for arsenic trioxide nanoparticles uptake rate also showed a positive correlation between the dosage, temperature, duration and trend; also explain MCF-7 to drugs possess active transport process.