Activation And Therapeutic Implications Of ATP-citrate Lyase In Human Breast Cancer

Objective:1. Explore ATP citrate lyase in breast cancer tissues and adjacent tissues(normal breast tissue)and its possible clinical significance. 2. Research after ACLY-si RNA interference in breast cancer cells, its expression and the proliferation of cancer cells and related clinical significance.Methods: 1.Using Western Blotting explore the expression of ACLY、p-ACLY in breast cancer tissues and the adjacent tissues. 2. Using the immunohistochemical method to detect the p-ACLY expression in patients with breast cancer and compare with clinical stage, grade, ER, PR, Ki67, HER-2, etc..3. the cell transfection to inhibite the expression of ACLY in breast cancer cells.4. PCR, Western Blotting to detect the ACLY expression after transfection of breast cancer cells 5. Using flow cytometry, CCK8 to detect the proliferation and apotpsis of breast cancer cells.Results: 1. Compered with the normal tissures,the expression of ACLY and p-ACLY was significantly higher in tumor in patients with breast cancer. 2. p-ACLY expression is increased in breast cancer and its expression correlated with the clinical stage, grade, and so on; 3.The expression of ACLY decreased after ACLY-si RNA transfected in human breast cancer MCF-7 cells; 4. Breast cancer cell proliferation was inhibited when ACLY-si RNA transfected MCF-7 human breast cancer cells.Conclusion: Overexpression and activation of ACLY and phosphorylated ACLY were found in patients with breast cancer, and phosphorylated ACLY was found to be a statistically significant negative prognostic factor. Selective ACLY inhibition by ACLY-si RNA resulted in the inhibition of tumor cell growth in MCF-7 cells. Our findings suggest that ACLY plays a critical role in cancer progression and metabolic changes, and may serve as a novel therapeutic target in breast cancer.