| The migration and adhension of monocytes induced by chemokine is an important event in the occurrence of AS.CXCR7 is a new discoved receptor of chemokine SDF-1, it can promote stem cells, lymphocytes and tumor cells migration, adhension to endothelial cells and transfering across endothelial cells, recent studies have shown that CXCR7 plays an important role in monocyte /macrophage recruitment in the process of the occurrence of AS, but the mechanism is still not clear. Ox-LDL is one of the main incentives of AS, and studies have confirmed that ox-LDL can increase the CXCR4 expression in monocytes to promote the migration and adhension of monocytes, but if the ox-LDL can change the CXCR7 expression in monocytes to regulate the migration and adhension of monocytes is not clear. ObjectiveDetermine the effect of ox-LDL on the CXCR7 expression in monocytes, clarify the regulation of SDF-1 / CXCR7 in recruitment of monocytes and further expound the mechanism of AS, which will provide new targets for effective prevention and control of AS. MethodsThe concentration and time effect of ox-LDL on CXCR7 m RNA expression was detected by RT-PCR in monocytes, use transwell and static endothelial monolayer adhesion experiment to investigate the effect of different concentration of ox-LDL on monocytes migration, adhension to endothelial cells and transfering across endothelial cells induced by SDF-1, in order to further confirm that up-regulation of CXCR7 expression could promote monocytes migration, adhension to endothelial cells and transfering across endothelial cells, we constructed CXCR7 over-expressed lentivirus vector upregulating CXCR7 expression in monocytes and verified by western blot to investigate if up-regulation of CXCR7 expression could promote monocytes migration,adhension to endothelial cells and transfering across endothelial cells. ResultsOx-LDL could promote the CXCR7 expression in monocytes, which 50ug/ml ox-LDL was most obvious and up-regulation of CXCR7 was monitored within 6h, peaked at 12 h. After treated with different concentration of ox-LDL for 48 h, monocytes were induced by 100ng/ml SDF-1, we found that ox-LDL increased monocytes migration, adhension to endothelial cells and transfering across endothelial cells in a concentration dependent manner. After transfected with CXCR7 over-expressed and control lentivirus vector, monocytes were induced by 100ng/ml SDF-1, we found that the former dramatically increased monocytes migration, adhension to endothelial cells and transfering across endothelial cells than the references. ConclusionsSDF-1/CXCR7 participates in monocytes migration, adhension to endothelial cells and transfering across endothelial cells, which are enhanced by ox-LDL; Ox-LDL up-regulates CXCR7 expression. |
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