Experimental Study Of Supply Blood To Free Flap With Cryopreserved Allograft Vascular Transplantation

Objective: This study was designed to supply blood to free flap with cryopreserved allograft vascular transplantation. The survival rate of the flaps was observed to assess the patency of the vessels after cryopreserved allograft vascular transplantation compared with fresh autologous vascular transplantation and fresh allograft vascular transplantation. To intestigate the viability to supply blood to free flap with cryopreserved allograft vascular transplantation in order to provide the experiment basis for supplying blood to free flap.Methods: 60 adult New Zealand white rabbits of either gender that weigh about 2.5 kg were randomly devided into three group with 20 rabbits in each group. To establish a femoral artery-defect model, abdominal flap with an area of 5×4cm2 which took superficial epigastric artery as the vascular pedicle was cut off and femoral artery with an length of 15 mm was taken between the proximal end of femoral artery and the initiating terminal. The vessel defect was bridged with fresh autologous vascular transplantation with an length of 15 mm in group A, fresh allograft vascular transplantation with an length of 15 mm in group B and cryopreserved cryopreserved allograft vascular transplantation with an length of 15 mm in group C under the optical microscope. Anti-inflammatory, anticoagulation and anti-artery-spasm treatment were performed after the transplantation. After the vascular transplantation, the flap color, temperature and flap capillary hyperemia reaction were observed to judge whehther the flap survived. The survival rate of the flaps was observed to assess the patency of the vessels. The patency of the vessels was analyzed statistically at 1st, 2nd, 3rd, 5th, 7th, 14 th and 21 th days after the transplantation. These data among three kinds of groups are compared in pair for the standard P<0.05 considered statistically significant.Results: The patency rate was 100% after the completion of grafting. The indicators of the flaps were observed after the vascular transplantation, and no obvious infection was found. The survival rate of the flaps was decreased with the prolong of time. There were 2 cases, 2 cases, 1 case and 1 case death at 1st, 2nd, 3rd and 5th in group A, respectively. There were 8 cases, 4 cases, 2 cases, 2 case and 1 case death at 1st, 2nd, 3rd, 5th and 7th days in group B, respectively. There were 3 cases, 2 cases, 2 cases and 1 case death at 1st, 2nd, 3rd and 7th days in group C, respectively. After the transplantation, the patency of the vessels was 70.0%, 15.0% and 60.0% in group A, group B and group C, respectively. It is no significant difference between A and C(P>0.05). There was significant difference between B and A(P<0.05). Significant difference was found between B and C(P<0.05).Conclusions: The survival rate of the flaps which was supplied blood with cryopreserved allograft vascular transplantation is higher than that of fresh allograft vascular transplantation. There is no statistical difference between fresh autologous vascular transplantation and cryopreserved cryopreserved allograft vascular transplantation. These findings suggest there is the clinical feasibility to supply blood to free flap with cryopreserved allograft vascular transplantation...