An Experimental Study On Efficacy Of Yimeifu About Acute Degree Ⅱ β-ray Skin Radiation-induced Injury Wound Of SD Rats

Objective : To investigate the treatment efficacy of Yimeifu Activity Due To The Molecular Biology Dressings on acute Ⅱdegree β-ray radiation-induced injury on skin of SD rats by comparing the changes of blood capillary endothelial cells and markers integrinβ1 and K19 of ESCs.Method:102 normal rats of 2 to 3 months old, either. male. or female.,feeding five days to. adapt. to the environment.,weight 180 to 200 g,feed in single cage. Irradiated the bare skin of rats’ buttocks singly 11 minutes and 52 seconds with 6MeV. high energy electron. beam produced. by a linear. accelerator.,to build. animal models. of acute Ⅱdegree beta ray skin injury. wound, divided. into. 3 groups. randomly.: experimental group.,control group and blank. group..The experimental. group. treated with. Yimeifu immediately after the radiation,three times a day.The control group treated with Biafine immediately,three times a day.The blank group without treatment.1d, 3d, 5d,7d, 14 d, 21 d, 35 d after irradiation, took the wound tissue samples of 6 rats’ buttocks each time, fixed and embedded to make the pathological section. The local wounds were observed by naked eyes and histopathology technology. The epidermal stem cell surface markers integrinβ1 and K19 expression were detected by immunohistochemistry methods(SP method) and imagen-pro plus analysis.All data should be deal with SPSS18.0 statistical software ANOVA, p <0.05 was considered statistically significant.Result:1.Wound observation:The swelling and desquamation reaction of rats in blank group was found in 3 days after irradiation,and congestion erythema in topical wound.compared with blank group,similar reaction appeared in 5-7day in experimental and control group,but the reaction did not include scaling,and the color of erythema was lighter.With the lapse time,the color of erythema in blank group deepened, and formed moss-like necrotic ulcers scab.The wound healing time in blank group was delayed,the newborn epithelial tissue was gradual thickening and harden to contracture state,the surface of the wound was not flat,color was deepened,congestion and edema it was obviously that the hair growth sparse.The healing process in experimental and control group was similar to blank group,but the newborn epithelial tissue was relatively flat, the color was closed to normal tissue,and hair growth was better. 2. Histopathological observation:Compared with blank group,the blood capillary endothelial swelling was less severe in experimental and control group.In the experimental and control group,the proliferation of microvasular and epithelial cells was activity,inflammation was lighter.3. Immunohistochemical observation : The brown granules expression of K19 and integrinβ1.positive cells. could. be found. in both. three groups.,located in. basal. cell. layer. and around the hair follicles,the experimental group was the. strongest.At the same time point,IOD of ESCs markers integrinβ1 and K19 in another two group has statistical significance( p<0.05) for the blank group,IOD of positive cells was no statistical significance( p>0.05) between experimental group and control group.The express of integrinβ1 and K19 reached a peak in 14-21 days after irradiation,and the highest appeared in experimental group,then gradually reduced.Conclusion:The expression of ESCs markers integrinβ1 and K19 increased significantly and the newborn microvasular was proliferated actively with Ymeifu on acute Ⅱdegree beta ray skin injury wound. Yimeifu has definited efficacy on acute Ⅱdegree beta ray skin injury wound,the treatment time could be shorten,and the healing degree is higher and Yimeifu has the clinical use and spread value.