The Value Of Serum Ang/Tie2、VEGF In The Assessment Of Diabetic Nephropathy

Objective : To observe the regular of levels change of serum angiopoietin-1(Ang-1),angiopoietin-2(Ang-2) and receptor Tie-2 as well as vascular endothelia growth factor(VEGF) in the progerssion of diabetic nephropathy(DN), and investigate the importance of serum Ang/Tie-2、VEGF levels in DN. Methods:Sixty Chinese patients with type 2 diabetes were enrolled. According to their UAER diabetic patients were divided into three groups randomly: 20 patients with normoalbuminuric diabetic(DM1 group), 22 with microalbuminuric diabetic(DM2 group) and 18 proteinuric diabetic(DM3 group). A total of22 health control were selected( NC group). Serum Ang-1, Ang-2, Tie2,VEGF levels were detected by enzyme-linked immunosorbent assay(ELISA). At the same time, related biochemical indicator were tested.Results:1.The level of serum Ang-2, VEGF increased in DM1 compared with NC, serum Ang-2, VEGF levels increased in DM2 compared with DM1, and serum Ang-2, VEGF levels increased in DM3 compared with DM2. There was statistical difference among four groups(P<0.05).2.The level of serum Ang-1 increased in DM1 compared with NC, thelevel of serum Ang-1 low in DM2 compared with NC, the level of serum Ang-1 low in DM3 compared with DM2. There was not statistics significance in difference among the four groups(P>0.05).3.There was no statistical significance in differences of Tie2(P>0.05). 4.UAER had positive correlation with Serum Ang-2、VEGF(r=0.470,0.404;p<0.001).UAER had negative correlation with Serum Ang-1(r=-0.310;p<0.05).Ang-2 had positive correlation with serum VEGF 、 Tie2( r=0.450,0.725;p<0.001),Ang-1 had positive correlation with serum Tie2(r=0.571;p<0.001),Ang-1 had negative correlation with Serum Ang-2、VEGF(r=-0.497,-0.396;p<0.001,0.05). 5.Line to UAER level as the dependent variable, multiple stepwise regression analysis showed that:Ang-1、Ang-2、VEGF、creatinine entered regression equation. They were independent risk factors for UAER. Conclusion:serum Ang-1, Ang-2,Tie2 and VEGF levels are closely related with DN. They may participate in the development of DN and affect the process of DN. Detection of serum Ang/Tie2 and VEGF levels can provide a new serological basis to evaluate the severity for the condition of DN.They have potential clinical value.