Research On Peripheral T, B Cell Subsets And NK Cells In Different Immune Status And Hepatitis B Cirrhosis With Chronic HBV Infection

Objective:Discuss the percentage changes of peripheral T, B cell subsets and NK cells in chronic HBV infestors with different immune states and hepatitis B cirrhosis. Methods: Seventy-five cases of chronic HBV infesors including 20 cases with immune clearance state, 20 cases with immunodeficiency(inactive) state and 35 cases with cirrhosis, and 20 cases for control were selected in our study. Flow Cytometry was used to detect the percentages of peripheral T and B lymphocyte subsets and NK cells,when HBV-DNA loads were detected by FQ-PCR. And the differences of the groups were analyzed. Results:1.Comparing with the control group, CD4+T cells were decreased in other four groups(P<0.05). The sequence of CD4+T cells from high to low was control group, immunodeficiency group, immune clearance group, compensated cirrhosis group and decompensated cirrhosis group. 2.CD4+/CD8+ ratio and NK cell were lower, but CD8+T cell and B cell were higher in immune clearance group than control group(P<0.05). 3.Patients in immunodeficiency group had lower ratio of CD4+/CD8+ ratio and higher CD8+T cell than those in control group(P<0.05). 4.In the all groups, patients with decompensated cirrhosis showed highest ratio of CD4+ to CD8+ T cells and B cells, but lowest CD3+T, CD8+T and NK cells(P<0.05). 5. Comparing with the control group and HBV-DNA negative group, HBV-DNA positive group has lower CD3+T, CD4+T and NK cells and highest CD8+T cell(P<0.05). 6.HBV-DNA negative group and positive group have highest B cell than those in control group(P<0.05). Conclusions:There appears immune dysfunction appears in patients with chronic HBV infection. It has certain clinical value in understanding patients’ immune states and progression of disease by detecting peripheral blood lymphocyte subsets and NK cells which is related to HBV-DNA loads.