Ultrastructural Observations Of Telocytes Damage In Ratmodel Of Oviduct Endometriosis

Part 1 Construction of animal model of oviduct endometriosis in ratsObjective: Animal model of oviduct endometriosis in rats was constructed, and can be used for research on tubal factor infertility in endometriosis;Methods: 40 female Sprague-Dawley rats(250~300 g) were devided into two groups averagely. The autotransplantion model of endometriosis at the mesentery of oviduct of rats was used to construct the model, with the control model receiving only blank suture in corresponding site. Two months later, the rats were killed, endometriotic vesicles, oviduct inflammation, and characteristic endometriotic changes were presented in lesions of oviduct in light microscope. The changes of microenvironment in oviduct such as COX-2, i NOS, LPO and Estrogen was detected by immunofluorescence staining combined with confocal laser scanning microscopy;Results: 1.Two months later, vesicles presented in transplantation site of experimental rats, with thin cystic wall and transparent yellow cyst fluid, and mesentery of oviduct were invaded by vesicles. 2.The mucosal folds in lesions of oviduct were disappeared, and endometrial glands were found in muscular layer, with variable degrees of fibrosis, 85% samples were successfully constructed into desired model. 3.In experimental group, the levels of COX-2, i NOS, LPO and Estrogen of oviduct tissue were significantly higher than that of normal control group,with P value less than 0.05; Conclusions: The rat model of endometriosis at the mesentery of oviduct by this method, conforms to pelvic microenvironment change in patients with endometriosis,and can be used as an ideal animal model to research on EMs- TFI.Part 2 Ultrastructural observations of telocytes damage in rat model ofoviduct endometriosisObjective: Endometriosis(EMs) frequently complicated with infertility in clinic. The aim of this study was to observe ultrastructural alterations of telocytes, discuss potential consequences on structure and reproductive function of oviduct.Methods: Autotransplantation of Sprague Dawley rat model of EMs affected oviduct tissue was used for ultrastructure observation by transmission electron microscope, together with quantitative determination of inflammatory factors(i NOS, COX-2) by Western blot.Results: In EMs affected oviduct, telocytes distribution was relative sparse and even undetectable, with cytoplasmic dissolving and vacuolating; decreased organelles, swelling mitochondrions, and dilatation of rough endoplasmic reticulums. Gap junctions between telocytes and other cells were faint or disappeared, obviously different from the control group, together with significant increased i NOS and COX-2(both P < 0.05).Conclusions: In EMs affected oviduct, telocytes display multiple cellular damage and cell lose, which was supposed to induce by increased level of inflammatory factors. Telocytes damage might play important roles in structural and functional abnormalities of oviduct.