Analysis Of Cartilage Cell Kinase Signal Difference Of TCM Syndrome Differentiation And Typing Of Avascular Osteonecrosis Of Femoral Head

Typing of TCM syndrome type of avascular Osteonecrosis of Femoral Head has been the lack of strong and direct evidence to confirm, difficult to achieve the objective of the real meaning and quantization, and provide scientific basis for typing to study the TCM syndrome type of femoral head necrosis. The phenotype of chondrocytes of different syndromes in different, through the research on MAPKs signal differences, this study hopes to look the part of the solution to the essence of TCM syndrome type of avascular necrosis of the femoral head location.ObjectiveThe establishment of different syndromes of patients with osteonecrosis of the femoral head femoral cartilage cell in vitro culture system, using the technique of genomics and proteomics,to investigate the MAPKs signal differences of different syndromes of chondrocytes, dig out the common features associated with asyndrome markers, provide objective and quantitative basis for research on theessence and reasonableclassification of disease syndromes of traditional Chinese medicineavascular necrosis of femoral head.MethodsThe collection of avascular necrosis of the femoral head in patients with a total of 20cases, and in accordance with the blood stasis type, liver and kidney deficiency type of syndrome differentiation and packet. Patients undergoing total hip replacement, the collection of different syndromes of intraoperative cartilage, were culturedchondrocytes in vitro, detection and value-added and apoptosis. Changes of different syndrome analysis of MMP-1/-13mRNA type of articular cartilage cells usingreal-time quantitative PCR and Western blot method to detect MMP-1 and MMP-13expression of mRNA in different syndrome types and protein, and the establishment of the corresponding protein atlas. The MAPKs signal pathway in different syndrometype of articular cartilage cells in difference analysis of different syndromes of articular cartilage of MAPKs signal pathway proteins, including ERKI/2, JNK, and P38kinase expression and its phosphorylation state, functional assessment of MAPKs in different syndrome types performance. Access to biological information points andon the results of sorting through comparative analysis, factor analysis, chemometricstechniques such as image, expression of different types of biological informationclassification search, exploring the relationshipbetween the MAPKs signal difference and femoral head necrosis syndrome type of traditional Chinese medicine.ResultsMMP-1/-13RNA type of deficiency of liver and kidney in the group in the expression of target gene were significantly smaller than the convenience of blood stasis type group.This indicates that there is obvious difference between the two syndromes in gene expression. In mass spectrometry shows are non consistent protein.ConelusionThe experimental results suggest that the apparent gap, and provides a new idea for the further study of typing of TCM syndrome type of femoral head necrosis, andbecome a large sample of experimental research is carried out according to the "preexperiment".