Primary Study On The Effects Of Resveratrol On Invasion And Metastasis Of Gastric Cancer SGC-7901 Cells And Its Mechanism

Objective: The hedgehog(Hh) signaling pathway is vital to vertebrate development, the homeostatic process and tumorigenesis. Epithelial-mesenchymal transition(EMT) is a cellular process during which epithelial cells become mesenchymal-appearing cells, which in turn promotes cancer metastasis and invasion. Resveratrol is a natural polyphenolic compound found in grapes, a variety of berries, peanuts and other plants. Numerous studies have demonstrated that the Hh signaling pathway is able to regulate the EMT, and that resveratrol can suppress carcinoma invasion and metastasis. In addition, certain studies have indicated that resveratrol can inhibit the Hh signaling pathway and EMT in some tumor cells, however, the research that whether resveratrol can also inhibit the Hh signaling pathway and EMT in gastric cancer cells has not yet been reported. In the present study, we investigated the efficacy of resveratrol on invasion and metastasis of gastric cancer SGC-7901 cells in vitro, and revealed the possible mechanisms.Methods: Gastric cancer SGC-7901 cells were treated with resveratrol or cyclopamine at different concentrations. The proliferation inhibition effect of different concentration of resveratrol and cyclopamine for SGC-7901 cells were estimated by MTT assay, and then calculated the half inhibition rate(IC50) of each drug. The invasion and metastasis of the cells were observed by performing a cell scratch test. The expression of Gli-1, Snail, E-cadherin and N-cadherin was detected by reverse transcription polymerase chain reaction(RT-PCR) and western blotting.Results: The MTT assay indicated that the viability of the SGC-7901 cells was significantly decreased in a concentration-dependent manner following resveratrol and cyclopamine treatment. The IC50 values of resveratrol and cyclopamine for SGC-7901 cells after 48 h were(54.92±2.52) μmol/L and(26.14 ± 1.09) μmol/L. The cell scratch test showed slower cells invasion and metastasis in the resveratrol group. The RT-PCR and western blotting showed a decrease in Gli-1, Snail, N-cadherin expression, and an increase in E-cadherin expression in the resveratrol and cyclopamine group compared with the control group.Conclusion: This study demonstrated that resveratrol is able to inhibit the invasion and metastasis in gastric cancer SGC-7901 cells. It may be related with the inhibition of the hedgehog signaling pathway, and further inhibition of EMT in gastric cancer cells.