| Purpose For malignant tumor, it has always been difficult to conquer in the clinical work, and its principle in occurrence and evolution has been studied widely at present,and its treatment also is varied. But for the exact etiology, treatment and prognosis judgement, it is still difficult to have satisfactory results. More and more studies have proved that the tumor microenvironment plays an important role in occurrence and development of malignant tumors, and plenty of studies have been implemented in the tumor microenvironment,including regulatory T cells(Treg). Chemotaxis, infiltration and function change of Treg may be responsible for the immune escape of tumor cells and associated with the prognosis of patients with cancer. This research attempts to through the analysis the expression of regulatory T cells in patients with tongue cancer to explore its relationship between clinical pathology and prognosis. And provide a new strategy for clinical treatment. Methods Collected set of oral medical center hospital in Anhui province from January 2009 to January 2009 for tongue ulcer line between living tissue for pathological examination after diagnosis of tongue and neck joint radical surgery, with complete follow-up data of 39 patients with tongue cancer. S-P method is used to detect the expression of Foxp3+ T cells in tongue cancer. Adopt positive piece known as the positive control, PBS buffer instead of a resistance as a negative control. All the data analysis by SPSS16.0 statistical software.Foxp3+ Tregs correlation analysis between cells and clinical pathological factors using chi-square test. Involving the survival time(overall survival, OS) and single factor survival analysis using the method of Kaplan-Meier, survival test ues the log-rank test, multi-factor survival analysis ues the Cox proportional hazards model. Single factor analysis of data including age, sex, TNM stage, neck lymph node condition, histopathological grading index of clinical pathology, etc. Multiple factors analysis is to the index of clinical pathology and single factor analysis of P<0.05 relevant variables into the model. Results 1. The Foxp3+ T cells were not expressed in normal oral squamous epithelium,and there is a large number of Foxp3+ T cell infiltration in tissue specimens of tongue cancer. The distribution of Foxp3+ T cell is different in tongue cancer of high, medium and low differentiation group(P = 0.036).There is no significant difference in the express of Foxp3+ T cell in different sex, age, TNM stage and lymph node status(P value was 0.455, 0.882, 0.199 and 0.455, respectively) 2. The high expression of Treg in patients with tongue carcinoma average survival time was 64.524±2.699(month), lower expression of Treg in patients with an average survival time was 50.500±4.600. Comparing the two groups(P = 0.012). The survival curves of high expression of Treg also reflects the tongue cancer survival time significantly shortened, high expression and low expression group five year survival rates were 44.44% and 85.71% respectively. 3. The average survival time in different age groups were 55.667±4.304 and 60.833±3.308respectively(P = 0.956); Men and women average survival time were 61.000±3.808 and 55.773±3.942 respectively(P = 0.473). Survival curves also reflect patients with different age, sex, living conditions have no obvious difference. 4. The tongue cancer patients, with or without lymph node metastasis patients survival time were 62.839±2.334 and 39.500±7.155(P = 0.001); In T1,2,the average survival time was 64.421±2.916, 52.000±4.301 in T3,4(P = 0.014); In provide differentiated tissues were 66.238±1.826, 55.500±5.009 and 5.009 ±7.597(P < 0.001). Survival curves show that patients with lymph node metastasis, TNM stage T3,4 and more memory, the survival curve drops more apparent and with poorly prognosis. 5. High differentiation, lymph node metastases in patients with tongue cancer stage, T1,2 patients had a higher 5 years of survival rate, 85.71%, 73.33% and 84.21%,respectively. Conclusions 1. Normal oral squamous epithelium don’t express Foxp3+ T cells.Tongue cancer tissue Abnormal expression of Foxp3+ T cells. Tongue cancer tissue infiltration of Foxp3+ T cell count is closely related to the patients with histopathological form, and has no obvious correlation with the patient’s gender, age, TNM staging, lymph node status. 2. The number of Foxp3+ T cell infiltration in tongue cancer closely related with the prognosis of patients, high expression of Foxp3+ T cells of tongue cancer tissues indicates poor prognosis. 3. Multiple factors analysis found that Foxp3+ T cell count, histological differentiation, TNM stage and lymphatic metastasis can be used as a prognostic indicator of tongue cancer patients. Including the histologic differentiation, TNM stage and lymphatic metastasis, clinical characteristics can be used as independent indicator of tongue cancer prognosis. |
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