Effect Of Zhixueqing Capsuleon Level Of Blood Lipidsand Liver Protective In Hyperlipidemia Guinea Pigs

Objective:The aim of this paper is to investigate the effects ZXQ on level of serum lipids and hepatic function in the hyperlipidemia guinea pigs induced by high fat diet.The guinea pigs’ body-weight, hepatic function,liver histomorphology,antioxidative capability and inflammatory factors were to be determined. The study would give some reference to its development and clinical use.Methods:The 60 healthy guinea pigs were divided randomly into 6 groups: Control,Model,XZK,ZXQL,ZXQM and ZXQH group,with 10 guinea pigs in each group. Control group was fed on base forage,the other five groups were fed on high fat diet(0.5% cholestero1,10% lard,and 89.5% base forage). All the groups were allowed to drink water and eat freely last for 6 weeks.After 2 weeks, the guinea pigs were treated respectively by daily oral gavage with XZK 250mg/kg,or ZXQ(1.78g/kg,3.56 g /kg,7.12g/kg)for 4 weeks.After 4 weeks of treatment, guinea pigs were sacrificed to test the levels of TCHO, TG, LDL-C,HDL-C, FFA,AST, ALT, TBA, TBIL and T-SOD in serum;The expressions of IL-1β,IL-6,IL-10, ICAM-1and TNF-α in hepatic by western blot;The liver was taken to detect the wet weight,then to be fixed with 10% neutral buffered formalin solution and paraffin imbedded for HE straining.Results:1The effects of ZXQ on body-weight of the hyperlipidemia guinea pigs:The trend of body-weight changes showed that the body-weight of guinea pigs in model group increased more quickly than that in other groups.After 1 week,each group of animals fed high-fat diet significantly increased and compared with the normal group,there is a statistically significant difference(P<0.01).3 weeks later,the body-weight of guinea pigs in XZK group were decreased and began to increase after four weeks, but the growth rate slowed. The trend of body-weight changes showed that the body-weight of guinea pigs in ZXQL, ZXQM and ZXQH group changed between the model group and normal group.2 The effects of ZXQ on blood lipids of the hyperlipidemia guinea pigs:Compared with model group, the ZXQ decreased the levels of serum TCHO,TG,LDL-C,FFA and increased level of serum in HDL-C significantly(P<0.05,P<0.01).3 The effects of ZXQ on hepatic function of thehyperlipidemia guinea pigs:Compared with model group,the ZXQ decreasedthe levels of AST,ALT, TBIL,TBA in serum significantly(P<0.05,P<0.01).4 The Effect of ZXQ on serum T-SOD level of the hyperlipidemia guinea pigs:Compared with control group,the activity of serum T-SOD in model group was decreased significantly(P<0.01). Compared with model group, both XZK and ZXQ increased the activity of serum T-SOD significantly(P<0.05,P<0.01)5 The effects of ZXQ on the liver index of hyperlipidemia guinea pigs:Compared with control group,the liver index in model groups increased significantly(P<0.01).Compared with model group,the liver index in ZXQL and ZXQH groups decreased significantly(P<0.05).6 The Effect of ZXQ liver tissue morphology of the hyperlipidemia guinea pigs:HE staining results of liver tissue sections showed that liver steatosis of guinea pig in XZK and ZXQ group were improved, the lipid droplets relatively small size, low density, a clear majority of lobular structure of the liver cells and liver cells arranged in a relatively neat, but still shows a small amount of lipid droplet vacuoles, which ZXQH group improved the most obvious, clear structure lobule and hepatic sinusoid cells arranged in neat rows, no significant intracellular lipid droplet vacuoles, nuclear morphology, structure is normal.7 The effects of ZXQ on the relative expression of hepatic TNF-α, IL-6, IL-1β, ICAM-1, IL-10 in hyperlipidemia guinea pigs:Compared with model group,both XZK and ZXQ decreased the relative expression of hepatic TNF-α,IL-6,IL-1β,ICAM-1and increased the relative expression of hepatic IL-10 significantly(P<0.05, P<0.01).Conclusions:ZXQ can reduce the levels of blood lipid, and alleviate liver damage of the hyperlipidemia guinea pig by regulatory lipid, anti-inflammatory effects, antioxidative ability augmentation and inhibit the expression of inflammatory cytokines.