The Research Of The Cremanthodium Extract Anti-plateau Hypoxic And The Mitochondrial Protective Mechanism

Objectiveiln this paper, to studying the Cremanthodium Butanolate extract of simulated plateau environment the protective effects of hypoxia rats and mice, study the method of the protection effect of hypoxia and its mechanism.Method:1. choose with different solvent extracts of anit-hypoxic activity, to find the Best extracts, using the hypoxia model, to find the best dose,.2.Rats simulated plateau environment Hypoxia experiment. Rats were randomly divided into four groups:normal group, hypoxic model group, positive drug acetazolamide group, Cremanthodium n-butyl alcohol Extract group. The large low pressure oxygen tank was used as the simulation of 8000 m plateau hypoxia environment, lack of oxygen 12 hours after dosing. The data of oxygen effects on physiological and biochemical index of rats was recorded.3.mice simulated plateau environment Hypoxia experiment. Rats were randomly divided into four groups:normal group, hypoxic model group, positive drug acetazolamide group, Cremanthodium n-butyl alcohol Extract group. The large low pressure oxygen tank was used as the simulation of 8000 m plateau hypoxia environment, lack of oxygen 12 hours after dosing. By observation of pathological biopsy and electronic microscope,biopsy check the effects of Myocardial tissue and brain tissue of the hypoxia mice. Detection of antioxidant related indicators.4.Hypoxia mice simulated plateau environment, extraction of mitochondria in mice, measured the mitochondrial membrane potential, oxidative phosphorylation enzymes and the Krebs cycle enzyme activity, observe mitochondrial apoptosis, mitochondria fusion and splitting protein expression.Results:1.The Cremanthodium n-butyl alcohol extract parts is the anit-hypoxic active site2.The Cremanthodium n-butyl alcohol extract Can improve the hypoxia mice blood pressure, blood sugar, but it can’t improve oxygen oxygen levels in the blood of the mice and calcium ion concentration, also don’t work for the red blood cells and platelets in the blood. It has no obvious improve to hypoxia mice blood urea nitrogen, lactate dehydrogenase and alanine aminotransferase, aspertate aminotransferase, creatinine.3.By observed pathological and electron microscope slices, Cremanthodium n-butyl alcohol extract have a protective effect to the erebral cells and myocardial cells.Through oxidation index, Cremanthodium n-butyl alcohol extract can improve erebral cells tissue T-the AOC and the SOD gsh-px activity, reduced the level of MDA, H2O2, OH, NO, and can improve activity of SOD, CAT CK of myocardial cell, low under the level of MDA, H2O2, OH.4.By hypoxia mice enzymes involved in breathing and energy cycle in the mitochondria of detection, Cremanthodium n-butyl alcohol extract can protect the hypoxia mouse’s smyocardial cell membrane potential, chrysanthemum n-butyl alcohol extract can improve hypoxia mouse aconitase, pyruvate dehydrogenase, mitochondrial complex Ⅰ、Ⅱ、Ⅲ activity in mitochondria, reduce the malate dehydrogenase, alpha ketone glutaric acid dehydrogenase, mitochondrial complex V activity. In hypoxia mice cardiomyocytes mitochondria, Cremanthodium n-butylalcohol extract of mitochondrial respiratory chain and the Krebs cycle enzyme present positively related to the concentration of protection. In high concentrations, blue chrysanthemum n-butyl alcohol extract could significantly improve the hypoxia mice cardiomyocytes in mitochondrial aconitase, malate dehydrogenase, alpha ketone glutaric acid dehydrogenase, pyruvate dehydrogenase, mitochondrial complex I, mitochondrialcomplex Ⅱ、mitochondrial complex III, mitochondrial complex V’s activity, and in the middle dose and low dose effect is not obvious. Cremanthodium extract can up regulation wb expression’s of SOD,mitofusion-2,dynamine-1,and,nrf-2.Conclusion:1.The Cremanthodium n-butyl alcohol extract is plateau hypoxia, the effects of n-butanol part hypoxia resistance effect is better.1.Cremanthodium n-butyl alcohol extract resistance to hypoxia action has nothing to do with improving oxygen levels in the blood, and improve the body’s ability to resist free radical.3.Cremanthodium extract can protect the Krebs cycle and mitochondrial respiratory chain, key enzymes improve mitochondrial antioxidant capacity, reduce mitochondrial apoptosis, promoting the fusion and splitting of mitochondria.