| Objective: by observing the different intervention under the condition of different categories of Traumatic brain injury(TBI Traumatic brain injuries) neurons form and space distinctiveness of rats rats learning and memory ability to change and its nuclear transcription factor on neurons in the nf-kappa B(Nucl-ear transcription factor- Kappa B, the nf-kappa B), tumor necrosis factor alpha(Tumornecrosis factor alpha,TNF alpha expression and the effect of further discussed based on the clear phlegm blood and water solution of Jannaoyizhi capsule capsule of TBI neurons of rats morphological changes and the nf-kappa B, the expression of TNF-ɑ h ave intervention effect.Methods: select grade clean healthy SD rats(2 months), after A random number is divided into four groups, respectively(control group) and B group(model group), group C(Jannaoyizhi capsule group), group D(Naofkang group) within the group.A single set of according to brain tissue samples sequence is divided into subgroups such as A1 to A4;Groups are using the improved Feeney method [1] building(control will not be processing), group A: open the cranial cavity blow device make the cranial cavity, see afterepidural exposed scalp suture, only to give regular feeding;Group B: building after regular feeding, shall not handle;Group C: Jannaoyizhi capsule ca-psule drug delivery 3 times/day, configuration specification: 0.75g/ml mixedsuspension, dosing way: lavage, single drug doses: 1.5 g/kg,;Group D: regular feeding, within the brain slices to medicine 3 times/day, con-figuration specification: 0.09 g/ml mixed suspension, dosing way: lavage, singledose lavage: 1.2 g/kg, using water maze(Morris water maze)[2]the experiment observation and record before building and gives the correspondingintervention model rats after building in 1, 3, 5, 7days of spacedi-stinctiveness behavioral changes such as learning and memory abilit[2],and within the prescribed time points respectively in the feeding after 3rd days,5th days, 7th day a random death group of rats, and correspondin-g brain tissue samples, fixed in 4% paraformaldehyde 24 h immediat-ely, gradientalcohol dehydration, paraffin embedding, serial section(the thick-ness of 5 microns) five take 1 piece, HE staining, the microscopic obser-vation of radiography, part with TNF alpha and NF-κB immunohistochemical analysis.Results: the experiment building success, according to the NSS scores, all function of model rats of severe defects.The total death, 20 rats(mortality 20.0%).All rats brain function complete before building, building, on the first day all space distinctiveness of learning and memory ability, but also for the slow response to external stimuli, depressed, etc.;3rd days in addition to the group B improvement is not obvious, and the rest before each performance is improved;5 d group B is poor in other ca-tegories, but are better;5 D C, group D space distinctiveness of learn ingand memory ability and the reaction has obvious progress, is better than that of group B and group A.Data analysis showed that C, D group rats TNF-ɑ detection rate and the nf-kappa B positive cells in the early days(3rdday) significantly difference(P>0.05), two groups compared with group B TNF alpha and low nf-kappa B(P<0.05), there are differences;(5-7days)after injury in rats of group C TNF alpha and nf-kappa B expression level is A, B, D group,(P<0.05) there are differences, compared with group B.TNF alpha and nf-kappa B were significantly reduced(p<0.05), significantdifferences.HE staining 24h-3d visible bruises focal cells around t o see a lot of liquefaction necrosis, inflammatory cells infiltrat-ion;5-7d theinflammatory cell infiltration with glial cell hyperplasia,model group t-han other groups of serious;Nepal’s impregnation each dam-age around the stove 24 h cell edema, vacuoles degeneration, Nepal’s dyeing, shallowand fewer model group than other groups, according to the severity from highto low :B>A>D>C.Tunel apoptotic cells in situdetection in 24h-3d number continues to increase, most 3d,7d when compared with the previous fall, according to the severity from high to low:B>A>D>C.Conclusion: 1. The Jiannaoyizhi capsule can inhibit experimental TBI neurons in rats TNF alpha and nf-kappa B the excessive expression of play to the role of the intervention neurons form change;Suppress TNF-ɑ, reduce inflammatory reaction and neuron apoptosis, reach the role of interventionneurons form change;2. According to the study speculated that its possible mechanisms include: a. inhibits damage focal inflammatory reaction, b. by intervening in-the nf-kappa b excessive release, inhibition of TNF-ɑ and nf-kappa b excessive activation, inhibition of the waterfall reaction appeared, effectively protect nerve cells.3.The wisdom of glue brain evolution capsule can inhibit or reduce the inflammatory reaction, enhance the ability to resist damage and apoptosis of neurons and improving memory and learning ability. |
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