A Preliminary Research On The Quality Control Of The Processed Products Of Radix Aconitum Vilmoriniani

In this paper, the constituents were isolated by column chromatography over si Lica gel, alumina and RP-C18 as well as recrystallization. Eleven compounds were obtained from the chloroform extract. The structures were elucidated on the basis of spectral analysis and physicochemical properties, and they were identified as yunaconitine(1), 8-deacetyl-yunaconitine(2), Geniculatine C(3), vilmorrianine B(4), vilmorrianine C(5), vilmorrianine D(6), Talatisamine(7), isotalatizidine(8), β-daucosterol(9), β-sitosterol acetate(10), β-sitosterol(11). Among them, yunaconitine and 8-deacetyl-yunaconitine are the main chemical composition of the processed products of Radix Aconitum vilmoriniani.The methods of hot plate test and writhing test were used to evaluate the analgesic effect. Anti-inflammation action was observed by the models of auricle swelling. LD50 was determined by the method of Bliss. The experimental results show that yunaconitine and 8-deacetyl-yunaconitine had analgesia effect on the pain caused by hot-plate, but there were no statistically significant difference. The pain caused by acetic acid had obvious analgesic action. High and low dose of yunaconitine could significantly reduce the number of mice body torsion and extend the incubation period of pain in mice. The effect of 8-deacetyl-yunaconitine was remarkable only when in the high dose. Compared with solvent group, there were little differences in inhibiting effect of auricle swelling caused by dimethylbenzene, and anti-inflammatory action was not exact. The poisonousness of yunaconitine was nearly 20 times of 8-deacetyl-yunaconitine. Yunaconitine and 8-deacetyl-yunaconitine might be the drug for peripheral analgesic effect. The poisonousness of 8-deacetyl-yunaconitine was less than Yunaconitine, and the effect was remarkable to the pain caused by acetic acid and the security was higher.Based on the result of chemical constituents and pharmacological activities, an HPLC method was established to measure contents of yunaconitine and 8-edacetyl-yunaconitine from the processed products of radix aconitum vilmoriniani. The two compounds were measured by high performance liquid chromatography. There was good linearity in the range of 2.5-180.0 μg for yunaconitine and 8-edacetyl-yunaconitine and the correlation coefficients were 1.0000. The average recovery(n=6) for yunaconitine and 8-edacetyl-yunaconitine was 99.30% with RSD of 1.10% and 99.36% with RSD of 0.50%, respectively. The method was simple, accurate and fast, which could be used in the determination of the processed products of Radix Aconitum vilmoriniani. The draft of quality standard has been established for it.