Expression Of Eg5 And Ki-67 In Non Small Cell Lung Cancer And Its Clinical Significance

Lung cancer is the first cause of death of cancer in the world. The incidence and mortality of lung cancer are on the rise in recent years. In 2003 its morbidity was 1,200,000, and the mortality was 1,100,000 per year which published by WHO. To understand its molecular carcinogenesis and growth mechanism, in the development of lung cancer, is the basis for prevention and treatment of lung cancer.Eg5 is a mitotic kinesin which N terminal of the motor is highly conserved domains. It can hydrolyze ATP and move to the microtubule positive. It is the motor molecule which has ATP enzyme activity and movement characteristics. Expression of Eg5 protein is related with cell mitosis proportion, so that the protein level may be associated with tumor growth speed. A series of pre clinical and clinical findings also supported this conjecture. Saijo found that the expression of Eg5 in non small cell lung cancer was significantly higher than that of normal lung tissues, and the expression of Eg5 in non small cell lung cancer patients with positive worse prognosis. Inhibitors of Eg5 can lead to apoptosis, which is the current research focus of tumor targeting therapy. Proliferating cell nuclear antigen Ki-67 is a protein associated with cell cycle, its function is closely related with mitosis, and it is indispensable in cell proliferation. We can understand cell proliferation activity of malignant tumor by detecting Ki-67 at present. Ki-67 expression is related with the carcinogenesis and development of lung cancer, and it is an adverse prognostic factor. Ki-67 has important reference value for diagnosis and prognosis of lung cancer.Objective: To investigate the relationship between expression of Eg5 and Ki-67 and pathological characteristics in non small cell lung cancer, and analyze the mechanism of Eg5 and Ki-67. Understanding the pathogenesis of lung cancer, and screened the high expression of Eg5 cases, selective giving Eg5 antagonist, which provide medical basis for lung cancer after surgery treatment.Methods: Retrospectively analyze surgical resection specimens of non small cell lung cancer from Handan Central Hospital in 2012-2013. Patients were not receiving preoperative radiotherapy or chemotherapy. We selected 87 cases of various types of non small cell lung cancer tissues and 20 cases of benign pulmonary disease(8 cases of pulmonary tuberculosis, 6 cases of bronchiectasis patients, 6 cases of pulmonary bulla patients) and away from the tumor tissue as negative control group. We successfully prepared three 5×8 size tissue microarray paraffin blocks(part of specimens were collected two sites), and 79 cases of effective samples and 20 cases of lung benign lesion specimens were obtained. Expressions of Eg5 and Ki-67 were detected by immunohistochemical staining. Gray value of positive cells was measured by HMIAS-2000 pathological image analysis system. We analyzed the relationship between Eg5 and Ki-67 expression and pathological characteristics in non small cell lung cancer, and the survival was followed up. All data were processed by SPSS16.0 statistical analysis software, and x2 test and correlation test were used. P<0.05 was considered as significant difference.Results:1 The positive rate of Eg5 was 64.6% in non small cell lung cancer. In various types of cancer tissues, the highest positive rate is adenosquamous carcinoma with 80%, and secondly adenocarcinoma with 71.4%. There was no statistically significant difference in all types of non small cell lung cancer tissues(X2=2.988, P>0.05). Expression of Eg5 protein was related with lymph node metastasis, the difference was statistically significant(X2=7.361, P<0.05). The expression of Eg5 was significantly related with the number of lymph node metastasis(X2=23.304, P<0.001).2 The expression of Eg5 protein was significantly correlated with the differentiation of non small cell lung cancer, stronger Eg5 expression in poor differentiation tissues. The difference was statistically significant(X2=6.127, P<0.05).3 The positive rate of Ki-67 was 64.6%, and no significant difference was found in all types of non small cell lung cancer(X2=6.974, P>0.05).4 The expression of Ki-67 protein was significantly related with lymph node metastasis in non small cell lung cancer(X2=10.298, P<0.05). In the analysis of lymph node metastasis, we found that Ki-67 expression was correlated with the number of lymph node metastasis(X2=8.468, P<0.05).5 In non small cell lung cancer, the expressions of Eg5 and Ki-67 were significantly positive correlation(correlation coefficient =0.0406, P<0.05).Conclusion:1 Eg5 protein was expressed in all types of non small cell lung cancer, and it was the highest in adenosquamous carcinoma, followed by adenoc-arcinoma. The expression of Eg5 protein was significantly related with lymph node metastasis in non small cell lung cancer, and its expression was markedly higher in lymph node metastasis group. The more the number pf lymph node metastasis, Eg5 expression increased, indicating poorer prognosis. In addition, there was significant difference between tumor differentiation and the expression of EG5 protein, and higher expression of Eg5 was found in poor differentiation tissues, which indicated poorer prognosis.2 Ki-67 proteins in various types of non-small cell lung cancer are expressed, and express the difference is not big, expression rate from 15% to 55%. And K-67 expression rate of lymph node metastasis group is significantly higher than without lymph node metastasis group. In the number of lymph node metastasis analysis shows that the more the number of lymph node metastasis, Ki-67 protein expression is higher, the worse the patient prognosis.3 There was positive correlation between expression of Eg5 and Ki-67, and correlation coefficient was 0.0406. The combined detection of Eg5 and Ki-67 can evaluate the prognosis of non small cell lung cancer patients.4 Eg5 is a molecular target, using Eg5 antagonists for neoadjuvant treatment can improve the therapeutic effect, reduce the pain patients, improve the survival of patients.5 The procedure of tissue microarray is simple, economical and con-venient, is a feasible and efficient technology method which can meet the need of related research and practical work.