The Intervention Effect Of Quercetin On B16 Murine Melanoma Cells Oxidative Stress Injury Induced By Hydrogen Peroxide

Objective:Vitiligo is a chronic skin disease characterized by portions of the skin losing their pigment, which can occur in any part of body,any sex,and any age. When skin lesions occur, they are most prominent on the face.The loss of skin pigmentation is particularly noticeable. Patients who are stigmatized for their condition may experience depression and similar mood disorders. There is no cure for vitiligo but many treatment options are available including topical steroids,calcineurin inhibitors,and phototherapy. Besides,a number of transplantation techniques has been developed by now,including transplantation of melanocyte precursors derived from hair follicles. Transplantation procedures are frequently used to treat segmental vitiligo which is poorly responsive to other types of treatment. Treatment is required twice a week for 6-12 months or longer. Because of the high doses of UVA,PUVA may cause side effects such as sunburn-type reactions or and melanomas. Therefore, the study of the treatment of vitiligo have great clinical value.The cause of vitiligo is unknown. Research suggests vitiligo may arise from autoimmune, genetic, oxidative stress, neural, or viral causes. In humans, oxidative stress is thought to be involved in vitiligo.Recently, many researches focus on oxidative stress as a pathogenesis of vitiligo, and many studies have confirmed the presence of oxidative stress in the skin lesions of patients with vitiligo. Oxidative stress can lead to dysfunction of melanocytes, become an important cause of vitiligo [2]. The study found that the skin lesions of patients with vitiligo accumulate a lot of hydrogen peroxide(Hydrogen peroxide, H2O2)[3-5]. Oxidative stress can cause the apoptosis of melanocytes,promote the occurrence and development of vitiligo.Quercetin(3,3’,4’,5,7-five dihydroxyflavone) is a flavonol found in many fruits, vegetables, leaves and grains. It can be used as an ingredient in supplements, beverages, or foods. Quercetin is a flavonoid widely distributed in nature. It is a kind of flavonoids that have a variety of biological activity,and have many kinds of medicinal value. Studies have shown that quercetin can remove free radicals, anti-inflammatory, anti-bacterial, anti-viral, anti-allergic, anti-tumor and anti-mutagenic, is popular at home and abroad in recent years.To investigate the potential protective effect of quercetin on the mice B16 melanocytes oxidative stress injury induced by hydrogen peroxide, providing a theoretical basis and laboratory basis for the repigmentation treatment of vitiligo.Methods:1 In this study, B16 melanoma cells were cultured in hydrogen peroxide with different concentrations,after which cell viability was determined by an MTS assay.Then building melanocyte oxidative stress model in vitro based on the above results.2 B16 melanoma cells were cultured in quercetin with different concentrations,after which cell viability was determined by an MTS assay.3 SOD activities and MDA levels assayed by the relevant indicators of oxidative stress testing kit.Results:1 B16 melanoma cells were treated with 1mmol/l H2O2 for 0.5h, the cell viability decreased significantly compared to the control group(P<0.01).2 B16 melanoma cells were pretreated with 25μmol/l,50μmol/l, 100μmol/l concentrations of qμercetin for 24 h,followed by exposure to 1mmol/l H2O2 for 0.5h.The cell viability of experimental group 1,2,3 increased significantly compared with the control group(P<0.05),but higher than the hydrogen peroxide group(P<0.05).3 SOD activities of experimental group 1,2,3 were significantly higher compared to the control group,but MDA levels decreased significantly(P<0.05).Conclusions:1 B16 melanoma cells viability were decreased with increasing of concentration of hydrogen peroxide.2 Quercetin can increase B16 melanoma cells viability in a dose-dependent manner within a certain range.3 Quercetin can increase cell viability of B16 in oxidative stress induced by H2O2.4 Through increasing the activity of SOD and reducing levels of MDA, quercetin can regulate the oxidative stress.