The Influence Of Sijunzitang And Its Demolition Party To The Biological Characteristics In Vitro Of Gastric Cancer SGC-7901, BGC-823 Cell Line Side Population Cells

Purpose: Selecting the side population cells of Gastric cancer SGC- 7901 and BGC-823 cell lines,detect the ability of proliferation,invasiveness and expression of apoptosis-related proteins of the two cell lines which were affected by Sijunzitang and its demolition party. To investigate the vitro biological mechanism of the side population cells affected by Sijunzitang.Method:1. Routinely cultured human gastric cancer cells, including SGC-7901(differentiated gastric cancer cell line)and BGC-823( poorly differentiated gastric cancer cell line).2. Sort out the side population cells of SGC-7901 and BGC-823 of gastric cancer cells3. Preparation the drug serum of Sijunzitang and its demolition parties.4. Observe the effect of Sijunzitang and its demolition party drug serum on the vitro proliferation of SP cells,non side popution(NSP)cells and unsorted cells by Tablet cloning experiment.5. Observe the effect of Sijunzitang and its demolition party drug serum on the invasiveness of SP cells, NSP cells and unsorted cells by Transwell experiment.6. Using Western blot detect the expreesion of apoptosis-related proteins Caspase-9、Cytochrome C、PRAP,which were interposed by drug serum.Result :1. The percentage of side group in gastric cancer cell line SGC-7901 is 2.68% ±0.58%.The percentage of side group in gastric cancer cell line BGC-823 is 3.28%±0.58%.2. After the intervention of Sijunzitang and its demolition party drug serum,the proliferation and invasiveness ability of SP cells,NSP cells and unsorted cells was inhibited in different level that comparing with normal saline group on the whole(p<0.05,individual group p>0.05). After the intervention of drug serum, the proliferation and invasiveness ability of SP cells is stronger than NSP cells’ on the whole(p<0.05,individual group p > 0.05).Sijunzitang inhibitory effect is stronger than its demolition party on the whole.(p<0.05,individual group p>0.05).3. After the intervention of Sijunzitang and its demolition party drug serum, there is a certain degree of improve in the expression of the apoptosis related protein Caspase-9, Cytochrome C, PRAP as compared with the control group with normal saline group on the whole; there is a certain degree of improve after 48 h of the intervention as compared with the 24 h on the whole;The expression of the SP generally lower than the group of the NSP;the ability of promotion of the expression of apoptosis related protein Caspase-9,Cytochrome C, PRAP of Sijunzitang is stronger than its demolition party on the whole.Conclusion:1. Drug serum of Sijunzitang and its demolition party can inhibit the proliferation and invasiveness of SP cells.2. Drug serum of Sijunzitang and its demolition can promote the expression of the apoptosis related protein Caspase-9, Cytochrome C, PRAP.3. Sijunzitang inhibitory effect of proliferation and invasiveness ability is stronger than its demolition party on the whole;Sijunzitang promot effect of the expression of the apoptosis related protein Caspase-9, Cytochrome C, PRAP is also stronger than its demolition party on the whole.