The Correlation Study Of Peripheral Blood CD4~+T Cells Balance And Parkinson’s Disease

Objective:Study of PD(Parkinson’s diseases, PD) in peripheral blood CD4+T cells and cells distribution and balance situation, explore the PD peripheral blood CD4+T cells and Th1 cells, Th2 cells, Th17 cells, Treg cells and Th1/Th2, of Th17/Treg and PD the relationship between the different stages.Method:To adopt case-control study, the selection of 45 patients with PD and healthy controls, 41 cases in venous blood was collected under the condition of informed consent.By Flow cytometry(Flow cytometry, FCM) detecting PD disease group of sex and age matched healthy controls in the peripheral blood CD4+T cell subgroup(Thl, Th2, Thl7, Treg), the percentage of and Th1/Th2, calculated the ratio of Th17/Treg.Analysis the correlation of each subgroup percentage and course.Results:1. PD in peripheral blood lymphocyte absolute value is lower than that of the control group(1.72、1.90, P>0.05);lymphocytes of white blood cells was lower than that of the control group(28.93、31.88, P<0.01). The PD group of CD4+T cells in peripheral blood of absolute value is lower than that of the control group(603.51、719.68, P>0.05); CD4+T cells accounted for the proportion of the absolute value of lymphocyte was lower than that of the control group(34.96、38.00, P<0.01).2. PD in the peripheral blood Thl cell subsets of CD4+T cells was higher than that of the control group(16.98、11.23, P<0.01), and UPDRS-III score in each group(PD severity) are positively correlated(P<0.01).3. PD in the peripheral blood Th2 cell subsets of CD4+T cells was lower than that of the control group(1.66、1.82, P<0.01), and UPDRS-III score in each group(PD severity) are negatively correlated(P<0.01).4. PD in the peripheral blood Th17 cell subsets of CD4+T cells was higher thanthat of the control group(0.87、0.52, P<0.01), but the score of UPDRS-III groups(PD severity) are positively correlated(P<0.01).5. PD in the peripheral blood Treg cell subsets of CD4+T cells was lower than that of the control group(4.12、5.91, P<0.01), and UPDRS-III score in each group(PD severity) are negatively correlated(P<0.01).6. PD in the peripheral blood Th1/Th2 cell subsets were higher than those in the control group(10.40、6.22, P<0.01), Th1/Th2 balanced to Th1 cell drift, and with UPDRS-III score(PD severity) are positively correlated(P<0.01).7. PD in the peripheral blood Th17/Treg cell subsets are higher than those in the control group(0.22、0.09, P<0.01), Th17/Treg balanced to Th17 cell drift, but each group with UPDRS-III score(PD severity) had no significant correlation(P>0.05).Conclusion:PD patients exist in the peripheral blood CD4+T T cells is lower than healthy controls, but no statistical significance;Th1 cells increased, reduce Th2 cells(p<0.01);Th1/Th2 balance to drift Th1 cells, the severity of the drift and U-Ⅲ score positive correlation;Treg cells is reduced, the increase in the number of Th17 cells of Th17/Treg drift to Th17 cells, the severity of the drift and U-Ⅲ score(disease severity)no significant correlation.