| Objective: 1. To investigate the expression of TSP-1 and VEGF in serum and tissue relations with primary liver cancer progression; 2. Compared with AFP, to evaluat the value of TSP-1 and VEGF for early diagnosis of liver cancer. 3. To investigatethe relationship of primary liver cancer with inflammatory cytokines of IL-1b, IL-6, IL-10, IL-12p70, IL-17 A, IL-22 and IL-23, then to evaluate the diagnosis significance of primary liver cancer.Method: 1. Collecte 88 cases of liver cancer serum samples and 53 cases of liver tissue sections, including 20 cases and 7 tissue sections of the first period of liver cancer, 25 cases and 18 tissue sections of the second period of liver cancer,43 cases and 28 tissue sections of the thirdperiod of liver cancer. serum collected 40 cases and 30 cases of cancer near normal liver tissue ashealthy control group.Randomly selected 51 cases, including 10 cases of the first period of liver cancer, 13 cases of the second period of liver cancer, 28 cases of the thirdperiod of liver cancer., the serum from the control group were randomly selected 29 cases were detected inflammatory Th17-related cytokines.2. The serum concentrations of TSP-1 and VEGF were detected by ELISA. 3. Liver tissue sections of TSP-1 and VEGF were detected by the methods of elivision plus. 4. The inflammatory cytokines IL-1b, IL-6, IL-10, IL-12p70, IL-17 A, IL-22, IL-23 was detected by Luminex x MAP technology,and the results was analyzed by Luminex 200 analysis software. 5. TSP-1 and VEGF between PHC patients and healthy control groups were compared using independent sample t test; Thecomparation of The first, second and third group of liver cancer with control group in serum use SNK-q test. Applicate ROC curves toget AUC area to assess the predictive power of the three indicators TSP-1, VEGF and AFP; Histological differences comparation use the linear trend test; Normal control group and PHC group of each cytokines comparation use independent samples t-test to analysis; Applicate the ROC curves to assess the predictive power of cytokines.All data were analyzed by SPSS19.0 statistical software, and we use P<0.05 as Inspection standards.Results : 1. TSP-1 serum concentrations of liver cancer was significantly lower than the control group(P <0.05); liver cancer serum VEGF concentration was significantly higher than the control group(P <0.05); 2. Serum concentrations of TPS-1of liver cancer group were lower than the control group, the differences were statistically significant(P <0.01), TPS-1 serum concentrations of the third liver cancer group lower than the first and the second liver cancer group, the difference was statistically significant(P <0.05), but there was no significant difference between the first and the second liver cancer group of TPS-1; and there was no significant difference between control group, the first and the second liver cancer group of VEGF(P > 0.05), but the third PHC serum VEGF concentrations higher than the normal control group, the first and the second liver cancer group, the differences were statistically significant(P <0.05); 3. Use serum concentrations of TSP-1, VEGF and AFP to make ROC curves, the area under the curve of TSP-1 was 0.893(P <0.05), AFP was 0.712(P <0.05), and VEGF was 0.568(P > 0.05). Descrip that TSP-1 and AFP as serum markers of liver cancer was statistically significant, and VEGF was limited. According to the size shown under the ROC curve TSP-1 diagnostic capabilities is better than AFP, and got higher sensitivity. 4. Use serum level of TSP-1 and AFP as variables to make ROC curve, analysis showed that TSP-1 and AFP as a marker for early diagnosis of liver cancer, were statistically significant, and VEGF for early diagnosis is not statistically significant. the area under the curve corresponding to TSP-1 and AFP were(0.883, P <0.05) and(0.821, P <0.05), the early diagnostic value of TSP-1 was better than AFP, In Youden index up to the standard selection TSP-1 cut-off point for132.11 ug / ml, the sensitivity was 95%, specificity was70.0%; select AFP truncation point 19.15 ng / ml, the sensitivity was 85.0%, specificity was 75.0%. 5. TSP-1 and VEGF were expressed in the cytoplasm of liver cancer cells and normal liver cells. In liver cancer cells and normal liver cells can be found in the expression of TSP-1, but the expression of the tumor cells is usually stronger than the normal liver cells. In liver cancer cells and liver cells can be found the expression of VEGF, but the expression of tumor cells generally stronger than within the liver cells. TSP-1 and VEGF expression of histologic progression and hepatocellular carcinoma are present correlation and linear relationship(P <0.05):The expression of TSP-1 was decling in PHC primary liver cancer with the progressing; The expression of VEGF was enhanced in PHC primary liver cancer with the progressing. 6. Independent sample t-test results,only IL-17 A were statistically significant between PHC and normal control group among 7 cytokines(P <0.05), the other six cytokines liver cancer group and control group was not statistically significant(P> 0.05) 7. Use IL-17 A serum concentrations to make ROC curve, evaluate thepossibility of cytokines as a liver cancer diagnostic marker. Its area under the curve AUCwas 0.882(P <0.05), described that IL-17 A as serum markers of liver cancer with statistical significance. Choose IL-17 A cut-off point for the 11.5ug/ml, the sensitivity was 68.6%, specificity was 93.1%Conclusion : 1. TSP-1concentration decreases with the progress of liver cancer, and VEGF concentration gradually increased, the expression of tissue was consistent; 2. TSP-1 have diagnostic value of liver cancer and early liver cancer. TSP-1 liver cancer diagnostic performance better than AFP; But the value of VEGF is very limited; 3. Among Th17 related inflammatory cytokines IL-1b, IL-6, IL-10, IL-12p70, IL-17 A, IL-22, IL-23, only IL-17 A have difference among liver cancer group and control group and the difference was statistically significant(P <0.05). ROC curve analysis showed that IL-17 A has the diagnostic value of liver cancer. |
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