| Objective:Study ginseng and astragalus hypoglycemic granule on early diabetic nephropathy rats kidney tissues the effect of ERK signal transduction pathway and protection to the kidneys and explore the ERK signal transduction pathway in position and its role in the development of early diabetic nephropathy, ginseng and astragalus hypoglycemic granule prevention mechanism of diabetic nephropathy, provide the experimental basis for clinical rational drug use.Methods:Choose SD male rats as experimental animals, random cent gives the normal control group(normal group) and diabetic nephropathy module(to build model rats left nephrectomy after 1 week, high sugar and high fat feed for 4 weeks, one-time intraperitoneal injection of urea with small dose of chain rhzomorph replication model of diabetic nephropathy).Building successful rats were randomly divided into diabetic nephropathy model(model group), Chinese medicine ginseng and astragalus hypoglycemic granule intervention group(hereinafter referred to as ginseng and astragalus group) and PD98059 positive control group(hereinafter referred to as PD98059 group), each group give corresponding intervention.Intervention in 8 weeks after testing each rat urine trace albumin excretion rate(UAER) and urine creatinine(available), fasting glucose, glycosylated hemoglobin, serum creatinine.ELISA method to detect fiber connections in the serum protein(FN) content;24 h urine was measured, and calculate the endogenous creatinine clearance rate(CCr), kidney weight/body weight;HE dyeing method to observe each kidney pathological form;Immunohistochemical method to detect groups of ERK protein involved in kidney tissue ERK signal transduction road, the expression of p- ERK and MKP 1.Results:1, in the aspect of blood sugar levels: compared with normal group, model group and PD98059 group were significantly higher(P < 0.01), there was no statistically significant difference between ginseng and astragalus group and normal group(P >0.05);Compared with model group, ginseng and astragalus group and PD98059 group were significantly lower(P < 0.01);Compared with PD98059 group, ginseng and astragalus group was lower than that in group PD98059(P < 0.01).2, in terms of available values: compared with normal group, ginseng and astragalus group, model group and PD98059 group were significantly increased(P < 0.01);Compared with model group, ginseng and astragalus group and PD98059 group were significantly lower(P < 0.01);Compared with PD98059 group, ginseng and astragalus group significantly lower than that of PD98059 group(P < 0.01).3, in terms of value of serum FN: compared with normal group, model group increased significantly(P<0.05), ginseng and astragalus, PD98059 group had no significant difference compared with normal group(P>0.05);Compared with model group, ginseng and astragalus group, PD98059 significantly reduced(P<0.01);And compared with PD98059,ginseng and astragalus group and the differences of no statistical significance(P>0.05).4, in terms of UAER value: compared with normal group, model group significantly increased(P<0.01), PD98059 also increased significantly(P<0.05),while the ginseng and astragalus group there was no statistically significant difference compared with normal group(P>0.05);Compared with model group, PD98059 and ginseng and astragalus group decreased significantly(P<0.01), and compared between PD98059 and ginseng and astragalus group there was no statistically significant difference(P>0.05).5, the kidney weight/body weight percentile:compared with normal group, model group, PD98059, ginseng and astragalus group were significantly increased(P<0.01);Group compared with model group, ginseng and astragalus was lower than that in model group(P=0.01), while PD98059 group compared with model group there was no statistically significant difference(P>0.05);Compared with PD98059 group, ginseng and astragalus group with no statistical difference(P>0.05).6, in terms of level of CCr: both two comparison there was nosignificant difference between groups(P>0.05).7, in the 24 h urine: compared with normal group, model group, PD98059 group were significantly greater than the normal group(P<0.01), compared with no significant difference between ginseng and astragalus group and normal group(P>0.05);Compared with model group, ginseng and astragalus group significantly less than the model group(P<0.05), there was no statistically significant difference between PD98059 group and model group(P>0.05);Compared with PD98059 group, ginseng and astragalus group significantly decreased(P<0.01), 8, in serum creatinine, uric creatinine concentration areas: both two comparison between groups was not meaningful conclusions.9 and HE staining were visible under normal group of normal rat kidney structure;Most of model group rats, see glomerular mesangial matrix mild nodular hyperplasia, part of the glomerulus see table with scattered in mesangial cell proliferation;Ginseng and astragalus group and the rat kidney pathological changes of PD98059 group were compared with model group light, only focal hyperplasia of glomerular mesangial matrix, some see glomerular segmental hyperplasia;Ginseng and astragalus group and PD98059 group rats compared with glomerular mesangial proliferation change has no obvious difference.Each rat kidney glomerular sclerosis was not found.10,immunohistochemical detection of each rat renal cortical tissue ERK protein involved in ERK signal transduction pathway, the expression of p- ERK and MKP 1 test results showed that normal rat kidney tissues without ERK, p- ERK protein expression and MKP- 1, the rest of the group rats glomerulus and renal tubule cells were expressed.Compared with model group, the expression of ginseng and astragalus group and PD98059 significantly reduce;Ginseng and astragalus group and the comparison between PD98059 group, no significant differences.Conclusion:Ginseng and astragalus hypoglycemic granule can reduce blood sugar, available through, serum FN, reduce urinary protein, inhibition of ERK protein involved in kidney tissue ERK signaling pathways, p- ERK and MKP 1 expression, thus inhibiting glomerular mesangial proliferation, inhibit glomerular fibrosis, protect renal function, prevention and treatment of diabetic nephropathy development. |
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