The Effect Of Chemotherapeutic Sensitivity Of Salvia Miltiorrhiza On Human Gastric Cancer SGC-7901 Cell

Objective:To observe the effect of Salvia miltiorrhiza on chemotherapy sensitivity of 5-Fu to tumor cells and its preliminary mechanism,and then to provide the basis for its application on chemotherapy when combined with anticancer drugs.Method:(1) The object of study and culture methods:Using the human gastric cancer SGC-7901 cells which were in logarithmic growth phase and grew in medium for the vitro experiment.(2) Intervention methods:using culture medium to preparat different concentration of Salvia miltiorrhiza,5-Fu and their mixed solution,in order to culture human gastric cancer cells in vitro;(3) The index selection and detection methods: observing the proliferation inhibition,apoptosis and TS, TP, DPD release of SGC-7901 cells. The inhibition proliferation of SGC-7901 cells was detected by MTT; the apoptosis of SGC-7901 cells was detected by flow cytometry; morphology was observed in Annexin V-FITC/PI staining under fluorescence microscope; adopt enzyme linked immunosorbent assay(ELISA method)to detect the TS, TP, DPD release of human gastric cancer SGC-7901 cells which were different drugs on;(4) Statistical methods: data were analyzed using statistical software SPSS17.0.Results:(1)Proliferation inhibition of the cells: The MTT results showed, high and middledose of non-toxic dose range of Salvia miltiorrhiza combined with 5-Fu are higher rate(P < 0.05)on proliferation of gastric cancer cells inhibition than the positive control group;(2)The apoptosis of cells: Flow cytometry results showed, non-toxic dose range of Salvia miltiorrhiza combined with 5-Fu can obviously increase the apoptosis rate of SGC-7901 cells.High middle and low dose of non-toxic dose range of Salvia miltiorrhiza combined with 5-Fu are higher rate(P < 0.05) on proliferation of gastric cancer cells apoptosis than the positive control group,from 32.54±0.58% increased to32.7±0.39%,38.3±1.61%(P<0.05) and 45.26±1.18%(P<0.01),and showed a concentration dependent;Using the Fluorescence microscopy,in cells climbing piece dyeing, we can find that the number of the intact cells in non-toxic dose range of Salvia miltiorrhiza combined with 5-Fu groups were all lower than the positive control group,the number of apoptotic cells increased with the drug concentration increasing;(3)The release of TS,TP,DPD: ELISA results showed that, 31.25, 62.5 and 125mg/L of Salvia miltiorrhiza combined with 5-Fu influence SGC-7901 for 48 h,the content of TS release from the positive control group as 67.03±1.75 pmol/L reduced to65.67±1.11,60.89±1.7 and 59.49±2.34 pmol/L; the release of TP from the positive control group as 11.46±0.39 pmol/L reduced to 10.76±0.71,8.21±1.12 and 8.58±0.73pmol/L; the release of DPD from the positive control group as 88.91±5.54 pmol/L reduced to 80.31±2.79、77.89±9.24 and 69.23±3.98 pmol/L.Conclusion:Salvia miltiorrhiza can enhance the inhibition of human gastric cancer SGC-7901 cell proliferation with the chemotherapy drugs, and accelerate the apoptosis. The mechanism of increased chemotherapy sensitivity of chemotherapeutic drugs may be associated with down regulation release of TS, TP, DPD on human gastric cancer cell line SGC-7901.