Effects Of IL-17F/IL-17RC On The Expression Of Caveolin-1 In Rat Pulmonary Microvascular Endothelial Cells

Objective To explore the expression of caveolin-1(Cav-1) induced by interleukin-17F/IL-17 receptor C(IL-17F/IL-17RC) in rat pulmonary microvascular endothelial cells(PMVECs).Methods Cultured PMVECs were divided into two groups of time-dependent experiment and IL-17 RC signal pathway intervention according to the results of time-dependent experimental group: namely peak time of IL-17F-induced Cav-1 and its phosphorylation(p-Cav-1) expression of PMVECs for formulating the incubation time between IL-17 F and PMVECs in IL-17 RC signal pathway intervention group. Time-dependent experiment group: Western blot was used to detect the expression of Cav-1 after IL-17 F stimulation for 0 h, 0.5 h, 1.5 h, 3 h, 6 h, 12 h, 24 h. Cav-1 and its phosphorylation expression after IL-17 F challenge for 0 min, 10 min, 30 min, 60 min, 90 min, 120 min were evaluated by Western blot. IL-17 RC signal pathway intervention group: PMVECs were divided into two groups after a 3-day pre-treatment of si RNA. The first group received a 60-min stimulation of 100 ng/ml IL-17 F before detecting the expression of p-Cav-1 while the second group was subject to a 24-hour stimulation of 100 ng/ml IL-17 F before detection. In addition, control, non-si RNA, IL-17RC-si RNA, IL-17 F and non-si RNA+IL-17 F groups were set as references for two groups respectively.Results IL-17 F up-regulated the expression of Cav-1 in a time-dependent manner. At 0 h, 0.5 h, 1.5 h, 3 h, 6 h, 12 h, 24 h, the relative expression levels of Cav-1 were(1.139 ± 0.134),(1.276 ± 0.166),(1.604 ± 0.080),(2.115 ± 0.231),(2.763 ± 0.226),(3.309 ± 0.493) and(3.963 ± 0.169). At 1.5 h, it was significantly higher than 0 h, peaked at 24 h and remained significantly higher than 0 h, 0.5 h, 1.5 h, 3 h, 6 h, 12 h(all P <0.05). And IL-17 F increased the expression of p-Cav-1 in a time-dependent manner. At 0 min, 10 min, 30 min, 60 min, 90 min, 120 min, the relative expression levels of p-Cav-1 were(0.540 ± 0.085),(0.880 ± 0.084),(1.437 ± 0.297),(1.491 ± 0.212),(1.017 ± 0.210) and(0.882 ± 0.074). At 10 min, p-Cav-1 was significantly higher than 0 min, peaked at 60 min, remained significantly higher than 0 min, 10 min, 30 min, 90 min, 120 min(all P < 0.05) and gradually decreased. At 120 min, it was still higher than 0 min. Compared with IL-17 F group, IL-17RC-si RNA significantly inhibited IL-17F-induced Cav-1 and its phosphorylation(2.126 ± 0.318 vs 3.897 ± 0.424 and 1.014 ± 0.136 vs 1.431 ± 0.298, all P < 0.05).Conclusions IL-17 F up-regulates the expressions of Cav-1 and p-Cav-1 in a time-dependent manner in PMVECs. And the silenced expression of IL-17 RC in PMVECs by IL-17RC-si RNA significantly inhibits the IL-17F-induced expressions of Cav-1 and p-Cav-1.