Chronic Manganese On Learning And Memory Ability And Hippocampal Bdnf And Apoptosis Protein Content

Objective To investigate the influence of different concentrations of manganese exposure on SD rats’hippocampus spatial learning and memory, brain-derived neurotrophic factor (BDNF) and apoptotic protein content.Methods 40 SD rats were randomly divided into four groups of 10 rats. Control group were intraperitoneally injected with saline. Low, medium and high does group were respetively intraperitoneally injected with 5 mg/kg,10 mg/kg,20 mg/kg of high temperature sterilization MnCl2.4H2O per day. The injection last for 18 weeks with 5 days a week. In the 6th,12th and 18th week of manganese staining, Morris water maze experiments were used to test the ability of learning and memory in rats. After 18 weeks’contamination, RT-PCR was used to detect levels of BDNF mRNA in the rats’hippocampus. Rats’ hippocampus Bax and Bcl2 protein expression were detected by Western blot.Results In the 6th,12th,18th week’s test, the traveled distance of the medium and high dose groups were longer than the control group; when exposed manganese to 12 and 18 weeks, the percentage target quadrant time of medium and high dose group were larger than the control group. The hippocampal BDNF mRNA content was negatively correlated with spatial memory ability. Chronic manganese makes the Bax up and Bcl-2 down.Conclusion Mediun and high dose of manganese chronic contamination can degrade spatial learning and memory in rats, along with the rise of Bax and decrease of bcl2 in the hippocampus, but not significantly change the content of BDNF.